Reversible thrombin detection by aptamer functionalized STING sensors

Signal Transduction by Ion NanoGating (STING) is a label-free technology based on functionalized quartz nanopipettes. The nanopipette pore can be decorated with a variety of recognition elements and the molecular interaction is transduced via a simple electrochemical system. A STING sensor can be ea...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2011-07, Vol.26 (11), p.4503-4507
Main Authors: Actis, Paolo, Rogers, Adam, Nivala, Jeff, Vilozny, Boaz, Seger, R. Adam, Jejelowo, Olufisayo, Pourmand, Nader
Format: Article
Language:English
Subjects:
Aptamer
Aptamers, Nucleotide
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
biosensors
Biotechnology
Blood Chemical Analysis - instrumentation
Blood Chemical Analysis - methods
Electrochemical Techniques
electrochemistry
Equipment Design
Fundamental and applied biological sciences. Psychology
Humans
Label-free
Nanopipette
Nanopore
Nanopores
Nanotechnology
oligonucleotides
quartz
Signal Transduction
STING
Thrombin
Thrombin - analysis
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Summary:Signal Transduction by Ion NanoGating (STING) is a label-free technology based on functionalized quartz nanopipettes. The nanopipette pore can be decorated with a variety of recognition elements and the molecular interaction is transduced via a simple electrochemical system. A STING sensor can be easily and reproducibly fabricated and tailored at the bench starting from inexpensive quartz capillaries. The analytical application of this new biosensing platform, however, was limited due to the difficult correlation between the measured ionic current and the analyte concentration in solution. Here we show that STING sensors functionalized with aptamers allow the quantitative detection of thrombin. The binding of thrombin generates a signal that can be directly correlated to its concentration in the bulk solution.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2011.05.010