ISsaga is an ensemble of web-based methods for high throughput identification and semi-automatic annotation of insertion sequences in prokaryotic genomes

Insertion sequences (ISs) play a key role in prokaryotic genome evolution but are seldom well annotated. We describe a web application pipeline, ISsaga (http://issaga.biotoul.fr/ISsaga/issaga_index.php), that provides computational tools and methods for high-quality IS annotation. It uses establishe...

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Bibliographic Details
Published in:Genome Biology 2011-03, Vol.12 (3), p.R30-R30, Article R30
Main Authors: Varani, Alessandro M, Siguier, Patricia, Gourbeyre, Edith, Charneau, Vincent, Chandler, Mick
Format: Article
Language:English
Subjects:
Biotechnology
Computational Biology
DNA Transposable Elements
Genetics
Genome, Archaeal
Genome, Bacterial
Internet
Life Sciences
Method
Molecular Sequence Annotation - methods
Open Reading Frames
Reproducibility of Results
Software
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Summary:Insertion sequences (ISs) play a key role in prokaryotic genome evolution but are seldom well annotated. We describe a web application pipeline, ISsaga (http://issaga.biotoul.fr/ISsaga/issaga_index.php), that provides computational tools and methods for high-quality IS annotation. It uses established ISfinder annotation standards and permits rapid processing of single or multiple prokaryote genomes. ISsaga provides general prediction and annotation tools, information on genome context of individual ISs and a graphical overview of IS distribution around the genome of interest.
ISSN:1474-760X
1465-6906
1474-760X
1465-6914
DOI:10.1186/gb-2011-12-3-r30