Generation of a monoclonal antibody reactive to prefusion myocytes

We established a novel monoclonal antibody, Yaksa that is specific to a subpopulation of myogenic cells. The Yaksa antigen is not expressed on the surface of growing myoblasts but only on a subpopulation of myogenin-positive myocytes. When Yaksa antigen-positive mononucleated cells were freshly prep...

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Bibliographic Details
Published in:Journal of muscle research and cell motility 2011-08, Vol.32 (1), p.31-38
Main Authors: Kurisaki, Tomohiro, Masuda, Aki, Nakagiri, Shiho, Hayata, Yoshihiro, Kuhara, Motoki, Kishi, Yoshiro, Sehara-Fujisawa, Atsuko
Format: Article
Language:English
Subjects:
Animal Anatomy
Animals
Antibodies, Monoclonal - isolation & purification
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cell Differentiation - immunology
Cell Differentiation - physiology
Cell Line
Female
Histology
Life Sciences
Mice
Mice, Inbred C57BL
Morphology
Muscle Cells - immunology
Muscle Development - immunology
Myoblasts - immunology
Myogenin - immunology
Original Paper
Proteomics
Rats
Rats, Wistar
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Summary:We established a novel monoclonal antibody, Yaksa that is specific to a subpopulation of myogenic cells. The Yaksa antigen is not expressed on the surface of growing myoblasts but only on a subpopulation of myogenin-positive myocytes. When Yaksa antigen-positive mononucleated cells were freshly prepared from a murine myogenic cell by a cell sorter, they fused with each other and formed multinucleated myotubes shortly after replating while Yaksa antigen-negative cells scarcely generated myotubes. These results suggest that Yaksa could segregate fusion-competent, mononucleated cells from fusion–incompetent cells during muscle differentiation. The Yaksa antigen was also expressed in developing muscle and regenerating muscle in vivo and it was localized at sites of cell–cell contact between mono-nucleated muscle cells and between mono-nucleated muscle cells and myotubes. Thus, Yaksa that marks prefusion myocytes before myotube formation can be a useful tool to elucidate the cellular and molecular mechanisms of myogenic cell fusion.
ISSN:0142-4319
1573-2657
DOI:10.1007/s10974-011-9247-8