Carboxyl-ebselen-based layer-by-layer films as potential antithrombotic and antimicrobial coatings

• Published in: Biomaterials 2011-11, Vol.32 (31), p.7774-7784
• Main Authors: Cai, Wenyi, Wu, Jianfeng, Xi, Chuanwu, Ashe, Arthur J, Meyerhoff, Mark E
• Format: Article
• Language: English
• Subjects: Advanced Basic Science; Animals; annealing; Anti-Infective Agents - chemistry; Anti-Infective Agents - pharmacology; Antimicrobial; Antithrombins - chemistry; Antithrombins - pharmacology; Azoles - chemistry; Azoles - pharmacology; bacteria; Biocompatibility; blood; Catalysis - drug effects; Coated Materials, Biocompatible - chemistry; Coated Materials, Biocompatible - pharmacology; coatings; Cross-Linking Reagents - pharmacology; crosslinking; Dentistry; Escherichia coli - cytology; Escherichia coli - drug effects; Free Radical Scavengers - pharmacology; medical equipment; Microbial Sensitivity Tests; Microbial Viability - drug effects; Microscopy, Electron, Scanning; Microscopy, Fluorescence; Nitric oxide; Nitric Oxide - chemical synthesis; Organoselenium Compounds - chemistry; Organoselenium Compounds - pharmacology; oxygen; Polyethyleneimine - pharmacology; polyurethanes; reducing agents; S-Nitrosothiols - chemistry; Selenium - chemistry; Sheep; soaking; Spectrophotometry, Ultraviolet; Superoxide; superoxide anion; Superoxides - chemistry; Thrombosis
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/j.biomaterials.2011.06.075

Abstract A carboxyl-ebselen-based layer-by-layer (LbL) film was fabricated by alternatively assembling carboxyl-ebselen immobilized polyethylenimine (e-PEI) and alginate (Alg) onto substrates followed by salt annealing and cross-linking. The annealed films exhibiting significantly improved stability are capable of generating nitric oxide (NO) from endogeneous S -nitrosothiols (RSNOs) in the presence of a reducing agent. The NO generation behaviors of different organoselenium species in solution phase are compared and the annealing mechanism to create stable LbL films is studied in detail. An LbL film coated polyurethane catheter is capable of generating physiological levels of NO from RSNOs even after blood soaking for 24 h, indicating potential antithrombotic applications of the coating. Further, the LbL film is also demonstrated to be capable of reducing living bacterial surface attachment and killing a broad spectrum of bacteria, likely through generation of superoxide (O2 − ) from oxygen. This type of film is expected to have potential application as an antithrombotic and antimicrobial coating for different biomedical device surfaces.