Feasibility of Treating Irradiated Bone with Intramedullary Delivered Autologous Mesenchymal Stem Cells

Background. We aimed to explore (i) the short-term retention of intramedullary implanted mesenchymal stem cells BMSCs and (ii) their impact on the bone blood flow and metabolism in a rat model of hindlimb irradiation. Methods. Three months after 30 Gy irradiation, fourteen animals were referred into...

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Bibliographic Details
Published in:BioMed research international 2011-01, Vol.2011 (2011), p.1-9
Main Authors: Phulpin, Bérengère, Dolivet, Gilles, Marie, Pierre-Yves, Poussier, Sylvain, Huger, Sandrine, Bravetti, Pierre, Graff, Pierre, Merlin, Jean-Louis, Tran, Nguyen
Format: Article
Language:English
Subjects:
Animals
Antigens, CD
Antigens, CD - metabolism
Bone Marrow Cells
Bone Marrow Cells - chemistry
Bone Marrow Cells - cytology
Bone Marrow Cells - metabolism
Cancer
Feasibility Studies
Gamma Rays
Indium
Life Sciences
Mesenchymal Stem Cell Transplantation
Mesenchymal Stem Cells
Mesenchymal Stromal Cells - chemistry
Mesenchymal Stromal Cells - cytology
Osteoblasts
Osteoblasts - chemistry
Osteoblasts - metabolism
Radiotherapy
Rats
Rats, Wistar
Regional Blood Flow
Statistics, Nonparametric
Tibia
Tibia - blood supply
Tibia - metabolism
Tibia - radiation effects
Tibia - surgery
Tomography, Emission-Computed, Single-Photon
Transplantation, Autologous
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Summary:Background. We aimed to explore (i) the short-term retention of intramedullary implanted mesenchymal stem cells BMSCs and (ii) their impact on the bone blood flow and metabolism in a rat model of hindlimb irradiation. Methods. Three months after 30 Gy irradiation, fourteen animals were referred into 2 groups: a sham-operated group (n= 6) and a treated group (n= 8) in which 111In-labelled BMSCs (2×106 cells) were injected in irradiated tibias. Bone blood flow and metabolism were assessed by serial T99mc-HDP scintigraphy and 1-wk cell retention by recordings of T99mc/111In activities. Results. The amount of intramedullary implanted BMSCs was of 70% at 2 H, 40% at 48 H, and 38% at 168 H. Bone blood flow and bone metabolism were significantly increased during the first week after cell transplantation, but these effects were found to reduce at 2-mo followup. Conclusion. Short-term cell retention produced concomitant enhancement in irradiated bone blood flow and metabolism.
ISSN:1110-7243
2314-6133
1110-7251
2314-6141
DOI:10.1155/2011/560257