Identification of APN2, the Saccharomyces cerevisiae homolog of the major human AP endonuclease HAP1, and its role in the repair of abasic sites

Abasic (AP) sites arise in DNA through spontaneous base loss and enzymatic removal of damaged bases. APN1 encodes the major AP-endonuclease of Saccharomyces cerevisiae. Human HAP1 (REF1) encodes the major AP endonuclease which, in addition to its role in DNA repair, functions as a redox regulatory p...

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Bibliographic Details
Published in:Genes & development 1998-10, Vol.12 (19), p.3137-3143
Main Authors: Johnson, R E, Torres-Ramos, C A, Izumi, T, Mitra, S, Prakash, S, Prakash, L
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Carbon-Oxygen Lyases - chemistry
Carbon-Oxygen Lyases - genetics
Deoxyribonuclease IV (Phage T4-Induced)
DNA Repair
DNA-(Apurinic or Apyrimidinic Site) Lyase
Fungal Proteins - chemistry
Fungal Proteins - genetics
Genes, rev
Humans
Molecular Sequence Data
Mutation
Research Paper
Saccharomyces cerevisiae
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae Proteins
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Summary:Abasic (AP) sites arise in DNA through spontaneous base loss and enzymatic removal of damaged bases. APN1 encodes the major AP-endonuclease of Saccharomyces cerevisiae. Human HAP1 (REF1) encodes the major AP endonuclease which, in addition to its role in DNA repair, functions as a redox regulatory protein. We identify APN2, the yeast homolog of HAP1 and provide evidence that Apn1 and Apn2 represent alternate pathways for repairing AP sites. The apn1Delta apn2Delta strain displays a highly elevated level of MMS-induced mutagenesis, which is dependent on the REV3, REV7, and REV1 genes. Our findings indicate that AP sites are highly cytotoxic and mutagenic in eukaryotes, and that the REV3, REV7-encoded DNA polymerase zeta mediates the mutagenic bypass of AP sites.
ISSN:0890-9369
1549-5477
DOI:10.1101/gad.12.19.3137