Identification of APN2, the Saccharomyces cerevisiae homolog of the major human AP endonuclease HAP1, and its role in the repair of abasic sites

Abasic (AP) sites arise in DNA through spontaneous base loss and enzymatic removal of damaged bases. APN1 encodes the major AP-endonuclease of Saccharomyces cerevisiae. Human HAP1 (REF1) encodes the major AP endonuclease which, in addition to its role in DNA repair, functions as a redox regulatory p...

Full description

Saved in:
Bibliographic Details
Published in:Genes & development 1998-10, Vol.12 (19), p.3137-3143
Main Authors: Johnson, R E, Torres-Ramos, C A, Izumi, T, Mitra, S, Prakash, S, Prakash, L
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Carbon-Oxygen Lyases - chemistry
Carbon-Oxygen Lyases - genetics
Deoxyribonuclease IV (Phage T4-Induced)
DNA Repair
DNA-(Apurinic or Apyrimidinic Site) Lyase
Fungal Proteins - chemistry
Fungal Proteins - genetics
Genes, rev
Humans
Molecular Sequence Data
Mutation
Research Paper
Saccharomyces cerevisiae
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae Proteins
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c415t-146de4c13e61cc3163a24d82e5c096b721e3a2f9a2b424fdc2a6bc25b1e48e993
cites cdi_FETCH-LOGICAL-c415t-146de4c13e61cc3163a24d82e5c096b721e3a2f9a2b424fdc2a6bc25b1e48e993
container_end_page 3143
container_issue 19
container_start_page 3137
container_title Genes & development
container_volume 12
creator Johnson, R E
Torres-Ramos, C A
Izumi, T
Mitra, S
Prakash, S
Prakash, L
description Abasic (AP) sites arise in DNA through spontaneous base loss and enzymatic removal of damaged bases. APN1 encodes the major AP-endonuclease of Saccharomyces cerevisiae. Human HAP1 (REF1) encodes the major AP endonuclease which, in addition to its role in DNA repair, functions as a redox regulatory protein. We identify APN2, the yeast homolog of HAP1 and provide evidence that Apn1 and Apn2 represent alternate pathways for repairing AP sites. The apn1Delta apn2Delta strain displays a highly elevated level of MMS-induced mutagenesis, which is dependent on the REV3, REV7, and REV1 genes. Our findings indicate that AP sites are highly cytotoxic and mutagenic in eukaryotes, and that the REV3, REV7-encoded DNA polymerase zeta mediates the mutagenic bypass of AP sites.
doi_str_mv 10.1101/gad.12.19.3137
format article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_317187</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17137101</sourcerecordid><originalsourceid>FETCH-LOGICAL-c415t-146de4c13e61cc3163a24d82e5c096b721e3a2f9a2b424fdc2a6bc25b1e48e993</originalsourceid><addsrcrecordid>eNpVkcFu1DAQQC0EKkvhyg3JJ05N6rETZ33gsKqAVqqgUsvZmjiTjaskXuykUv-CT25CVxWcLNnvjS0_xj6CyAEEnO-xyUHmYHIFqnrFNlAWJiuLqnrNNmJrRGaUNm_Zu5TuhRBaaH3CTkylSwlqw_5cNTROvvUOJx9GHlq-u_khz_jUEb9F5zqMYXh0lLijSA8-eSTehSH0Yb_SKzfgfYi8mwccF5vT2IRxdj1hIn65u4EzjmPD_ZR4DD1xP_61Ih3Qx3UG1pi848lPlN6zNy32iT4c11P269vXu4vL7Prn96uL3XXmCiinDArdUOFAkQbnFGiFsmi2kkonjK4rCbTstAZlXciibZxEXTtZ1kDFloxRp-zL89zDXA_UuOUXIvb2EP2A8dEG9Pb_k9F3dh8erIIKttXifz76MfyeKU128MlR3-NIYU52oVS1BFrA_Bl0MaQUqX25A4RdE9oloQVpwdg14SJ8-vdlL_ixmXoCmlaZlw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17137101</pqid></control><display><type>article</type><title>Identification of APN2, the Saccharomyces cerevisiae homolog of the major human AP endonuclease HAP1, and its role in the repair of abasic sites</title><source>Open Access: PubMed Central</source><source>Freely Accessible Science Journals - check A-Z of ejournals</source><creator>Johnson, R E ; Torres-Ramos, C A ; Izumi, T ; Mitra, S ; Prakash, S ; Prakash, L</creator><creatorcontrib>Johnson, R E ; Torres-Ramos, C A ; Izumi, T ; Mitra, S ; Prakash, S ; Prakash, L</creatorcontrib><description>Abasic (AP) sites arise in DNA through spontaneous base loss and enzymatic removal of damaged bases. APN1 encodes the major AP-endonuclease of Saccharomyces cerevisiae. Human HAP1 (REF1) encodes the major AP endonuclease which, in addition to its role in DNA repair, functions as a redox regulatory protein. We identify APN2, the yeast homolog of HAP1 and provide evidence that Apn1 and Apn2 represent alternate pathways for repairing AP sites. The apn1Delta apn2Delta strain displays a highly elevated level of MMS-induced mutagenesis, which is dependent on the REV3, REV7, and REV1 genes. Our findings indicate that AP sites are highly cytotoxic and mutagenic in eukaryotes, and that the REV3, REV7-encoded DNA polymerase zeta mediates the mutagenic bypass of AP sites.</description><identifier>ISSN: 0890-9369</identifier><identifier>EISSN: 1549-5477</identifier><identifier>DOI: 10.1101/gad.12.19.3137</identifier><identifier>PMID: 9765213</identifier><language>eng</language><publisher>United States: Cold Spring Harbor Laboratory Press</publisher><subject>Amino Acid Sequence ; Carbon-Oxygen Lyases - chemistry ; Carbon-Oxygen Lyases - genetics ; Deoxyribonuclease IV (Phage T4-Induced) ; DNA Repair ; DNA-(Apurinic or Apyrimidinic Site) Lyase ; Fungal Proteins - chemistry ; Fungal Proteins - genetics ; Genes, rev ; Humans ; Molecular Sequence Data ; Mutation ; Research Paper ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - enzymology ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae Proteins</subject><ispartof>Genes &amp; development, 1998-10, Vol.12 (19), p.3137-3143</ispartof><rights>Copyright © 1998, Cold Spring Harbor Laboratory Press 1998</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-146de4c13e61cc3163a24d82e5c096b721e3a2f9a2b424fdc2a6bc25b1e48e993</citedby><cites>FETCH-LOGICAL-c415t-146de4c13e61cc3163a24d82e5c096b721e3a2f9a2b424fdc2a6bc25b1e48e993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC317187/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC317187/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9765213$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Johnson, R E</creatorcontrib><creatorcontrib>Torres-Ramos, C A</creatorcontrib><creatorcontrib>Izumi, T</creatorcontrib><creatorcontrib>Mitra, S</creatorcontrib><creatorcontrib>Prakash, S</creatorcontrib><creatorcontrib>Prakash, L</creatorcontrib><title>Identification of APN2, the Saccharomyces cerevisiae homolog of the major human AP endonuclease HAP1, and its role in the repair of abasic sites</title><title>Genes &amp; development</title><addtitle>Genes Dev</addtitle><description>Abasic (AP) sites arise in DNA through spontaneous base loss and enzymatic removal of damaged bases. APN1 encodes the major AP-endonuclease of Saccharomyces cerevisiae. Human HAP1 (REF1) encodes the major AP endonuclease which, in addition to its role in DNA repair, functions as a redox regulatory protein. We identify APN2, the yeast homolog of HAP1 and provide evidence that Apn1 and Apn2 represent alternate pathways for repairing AP sites. The apn1Delta apn2Delta strain displays a highly elevated level of MMS-induced mutagenesis, which is dependent on the REV3, REV7, and REV1 genes. Our findings indicate that AP sites are highly cytotoxic and mutagenic in eukaryotes, and that the REV3, REV7-encoded DNA polymerase zeta mediates the mutagenic bypass of AP sites.</description><subject>Amino Acid Sequence</subject><subject>Carbon-Oxygen Lyases - chemistry</subject><subject>Carbon-Oxygen Lyases - genetics</subject><subject>Deoxyribonuclease IV (Phage T4-Induced)</subject><subject>DNA Repair</subject><subject>DNA-(Apurinic or Apyrimidinic Site) Lyase</subject><subject>Fungal Proteins - chemistry</subject><subject>Fungal Proteins - genetics</subject><subject>Genes, rev</subject><subject>Humans</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Research Paper</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - enzymology</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae Proteins</subject><issn>0890-9369</issn><issn>1549-5477</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNpVkcFu1DAQQC0EKkvhyg3JJ05N6rETZ33gsKqAVqqgUsvZmjiTjaskXuykUv-CT25CVxWcLNnvjS0_xj6CyAEEnO-xyUHmYHIFqnrFNlAWJiuLqnrNNmJrRGaUNm_Zu5TuhRBaaH3CTkylSwlqw_5cNTROvvUOJx9GHlq-u_khz_jUEb9F5zqMYXh0lLijSA8-eSTehSH0Yb_SKzfgfYi8mwccF5vT2IRxdj1hIn65u4EzjmPD_ZR4DD1xP_61Ih3Qx3UG1pi848lPlN6zNy32iT4c11P269vXu4vL7Prn96uL3XXmCiinDArdUOFAkQbnFGiFsmi2kkonjK4rCbTstAZlXciibZxEXTtZ1kDFloxRp-zL89zDXA_UuOUXIvb2EP2A8dEG9Pb_k9F3dh8erIIKttXifz76MfyeKU128MlR3-NIYU52oVS1BFrA_Bl0MaQUqX25A4RdE9oloQVpwdg14SJ8-vdlL_ixmXoCmlaZlw</recordid><startdate>19981001</startdate><enddate>19981001</enddate><creator>Johnson, R E</creator><creator>Torres-Ramos, C A</creator><creator>Izumi, T</creator><creator>Mitra, S</creator><creator>Prakash, S</creator><creator>Prakash, L</creator><general>Cold Spring Harbor Laboratory Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>19981001</creationdate><title>Identification of APN2, the Saccharomyces cerevisiae homolog of the major human AP endonuclease HAP1, and its role in the repair of abasic sites</title><author>Johnson, R E ; Torres-Ramos, C A ; Izumi, T ; Mitra, S ; Prakash, S ; Prakash, L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-146de4c13e61cc3163a24d82e5c096b721e3a2f9a2b424fdc2a6bc25b1e48e993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Amino Acid Sequence</topic><topic>Carbon-Oxygen Lyases - chemistry</topic><topic>Carbon-Oxygen Lyases - genetics</topic><topic>Deoxyribonuclease IV (Phage T4-Induced)</topic><topic>DNA Repair</topic><topic>DNA-(Apurinic or Apyrimidinic Site) Lyase</topic><topic>Fungal Proteins - chemistry</topic><topic>Fungal Proteins - genetics</topic><topic>Genes, rev</topic><topic>Humans</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Research Paper</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - enzymology</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Johnson, R E</creatorcontrib><creatorcontrib>Torres-Ramos, C A</creatorcontrib><creatorcontrib>Izumi, T</creatorcontrib><creatorcontrib>Mitra, S</creatorcontrib><creatorcontrib>Prakash, S</creatorcontrib><creatorcontrib>Prakash, L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Genes &amp; development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Johnson, R E</au><au>Torres-Ramos, C A</au><au>Izumi, T</au><au>Mitra, S</au><au>Prakash, S</au><au>Prakash, L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of APN2, the Saccharomyces cerevisiae homolog of the major human AP endonuclease HAP1, and its role in the repair of abasic sites</atitle><jtitle>Genes &amp; development</jtitle><addtitle>Genes Dev</addtitle><date>1998-10-01</date><risdate>1998</risdate><volume>12</volume><issue>19</issue><spage>3137</spage><epage>3143</epage><pages>3137-3143</pages><issn>0890-9369</issn><eissn>1549-5477</eissn><abstract>Abasic (AP) sites arise in DNA through spontaneous base loss and enzymatic removal of damaged bases. APN1 encodes the major AP-endonuclease of Saccharomyces cerevisiae. Human HAP1 (REF1) encodes the major AP endonuclease which, in addition to its role in DNA repair, functions as a redox regulatory protein. We identify APN2, the yeast homolog of HAP1 and provide evidence that Apn1 and Apn2 represent alternate pathways for repairing AP sites. The apn1Delta apn2Delta strain displays a highly elevated level of MMS-induced mutagenesis, which is dependent on the REV3, REV7, and REV1 genes. Our findings indicate that AP sites are highly cytotoxic and mutagenic in eukaryotes, and that the REV3, REV7-encoded DNA polymerase zeta mediates the mutagenic bypass of AP sites.</abstract><cop>United States</cop><pub>Cold Spring Harbor Laboratory Press</pub><pmid>9765213</pmid><doi>10.1101/gad.12.19.3137</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0890-9369
ispartof Genes & development, 1998-10, Vol.12 (19), p.3137-3143
issn 0890-9369
1549-5477
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_317187
source Open Access: PubMed Central; Freely Accessible Science Journals - check A-Z of ejournals
subjects Amino Acid Sequence
Carbon-Oxygen Lyases - chemistry
Carbon-Oxygen Lyases - genetics
Deoxyribonuclease IV (Phage T4-Induced)
DNA Repair
DNA-(Apurinic or Apyrimidinic Site) Lyase
Fungal Proteins - chemistry
Fungal Proteins - genetics
Genes, rev
Humans
Molecular Sequence Data
Mutation
Research Paper
Saccharomyces cerevisiae
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae Proteins
title Identification of APN2, the Saccharomyces cerevisiae homolog of the major human AP endonuclease HAP1, and its role in the repair of abasic sites
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T09%3A39%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20of%20APN2,%20the%20Saccharomyces%20cerevisiae%20homolog%20of%20the%20major%20human%20AP%20endonuclease%20HAP1,%20and%20its%20role%20in%20the%20repair%20of%20abasic%20sites&rft.jtitle=Genes%20&%20development&rft.au=Johnson,%20R%20E&rft.date=1998-10-01&rft.volume=12&rft.issue=19&rft.spage=3137&rft.epage=3143&rft.pages=3137-3143&rft.issn=0890-9369&rft.eissn=1549-5477&rft_id=info:doi/10.1101/gad.12.19.3137&rft_dat=%3Cproquest_pubme%3E17137101%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c415t-146de4c13e61cc3163a24d82e5c096b721e3a2f9a2b424fdc2a6bc25b1e48e993%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=17137101&rft_id=info:pmid/9765213&rfr_iscdi=true