Kinetics of messenger accumulation coding for IFN γ, related to modifications in the poly(A) RNA population of activated human lymphocytes

Exposure of human lymphocytes to a mitogen induces the appearance of newly synthesized RNAs and proteins. This study describes the changes in overall synthesis as measured by pulse labelling of PHA treated lymphocytes as well as a qualitative analysis of the protein synthetic patterns “in vivo” and...

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Bibliographic Details
Published in:Nucleic acids research 1984-03, Vol.12 (6), p.2629-2640
Main Authors: Vaquero, Catherine, Sanceau, Josiane, Sondermeyer, Paul, Falcoff, Rébéca
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cells, Cultured
Female
Fundamental and applied biological sciences. Psychology
Gene expression
Humans
Interferon-gamma - genetics
Kinetics
Lymphocyte Activation
Lymphocytes - immunology
Molecular and cellular biology
Molecular genetics
Nucleic Acid Hybridization
Oocytes - metabolism
Phytohemagglutinins
Poly A - genetics
Protein Biosynthesis
RNA - genetics
RNA, Messenger - genetics
RNA, Messenger - isolation & purification
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Summary:Exposure of human lymphocytes to a mitogen induces the appearance of newly synthesized RNAs and proteins. This study describes the changes in overall synthesis as measured by pulse labelling of PHA treated lymphocytes as well as a qualitative analysis of the protein synthetic patterns “in vivo” and “in vitro”. Both the levels of RNA and protein synthesis increase drastically in PHA stimulated cells, while cultures incubated without mitogen remained at background levels. The low translational activity in control cells is not due to the absence of messengers since the extracted RNAs clearly direct the synthesis of high molecular weight proteins when translated “in vitro”. A number of qualitative differences are seen in the “in vitro” translation of RNA extracted from induced and non-induced lymphocytes, although the apparent protein synthetic pattern “in vivo” remains identical. The secretion of IFN-γ is one of the newly expressed functions in stimulated lymphocytes and therefore has been studied more detailed in a time-course of the messenger level compared to the secreted activity of the medium. A specific probe was used to quantitate in Northern blot's the accumulation of mRNA coding for IFN- γ.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/12.6.2629