Energy substrate metabolism in fresh and stored human platelets

The latent capacity of human platelets for oxidizing several important energy-yielding substrates has been revealed by hypoosmolaric incubation conditions. The data show that the human platelet has a considerable capacity to oxidize both glucose and long-chain fatty acids. Long-chain fatty acids app...

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Bibliographic Details
Published in:The Journal of clinical investigation 1970-01, Vol.49 (1), p.119-127
Main Authors: Cohen, P, Wittels, B
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - pharmacology
Blood Platelets - metabolism
Blood Preservation
Carbon Isotopes
Carnitine - pharmacology
Chromatography, Thin Layer
Citric Acid Cycle
Fatty Acids - metabolism
Glucose - metabolism
Humans
In Vitro Techniques
Oleic Acids - metabolism
Osmolar Concentration
Oxidation-Reduction
Pyruvates - metabolism
Succinates - metabolism
Temperature
Time Factors
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Summary:The latent capacity of human platelets for oxidizing several important energy-yielding substrates has been revealed by hypoosmolaric incubation conditions. The data show that the human platelet has a considerable capacity to oxidize both glucose and long-chain fatty acids. Long-chain fatty acids appear to rank favorably with glucose as a potential energy substrate. In a number of mammalian tissues, (-)-carnitine serves to regulate the rate at which long-chain fatty acids are oxidized. Evidence was obtained which suggests that (-)-carnitine functions in a similar role in the platelet. After storage of human platelets at 4 degrees C for 24 hr, the oxidative capacity for glucose was reduced by approximately 25% and for long-chain fatty acids by almost 50%. Investigation of the component parts of the metabolic pathways indicated that a marked decrease in the capacity of the Krebs cycle could be responsible for the decrement in energy substrate oxidation.
ISSN:0021-9738
DOI:10.1172/JCI106210