Storage globulins pass through the Golgi apparatus and multivesicular bodies in the absence of dense vesicle formation during early stages of cotyledon development in mung bean

During seed development and maturation, large amounts of storage proteins are synthesized and deposited in protein storage vacuoles (PSVs). Multiple mechanisms have been proposed to be responsible for transporting storage proteins to PSVs in developing seeds. In this study, a specific antibody was r...

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Bibliographic Details
Published in:Journal of experimental botany 2012-02, Vol.63 (3), p.1367-1380
Main Authors: Wang, Junqi, Tse, Yu Chung, Hinz, Giselbert, Robinson, David G., Jiang, Liwen
Format: Article
Language:English
Subjects:
Antibodies
Biological and medical sciences
Cotyledon - metabolism
Cotyledons
Fabaceae - metabolism
Fundamental and applied biological sciences. Psychology
Globulins
Globulins - metabolism
Golgi Apparatus - metabolism
Multivesicular Bodies - metabolism
Plant cells
Plant physiology and development
Plants
Proteins
Receptors
RESEARCH PAPER
Research Papers
Seeds
Storage proteins
Vacuoles
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Summary:During seed development and maturation, large amounts of storage proteins are synthesized and deposited in protein storage vacuoles (PSVs). Multiple mechanisms have been proposed to be responsible for transporting storage proteins to PSVs in developing seeds. In this study, a specific antibody was raised against the mung bean (Vigna radiata) seed storage protein 8S globulin and its deposition was followed via immunogold electron microscopy in developing mung bean cotyledons. It is demonstrated that non-aggregated 8S globulins are present in multivesicular bodies (MVBs) in early stages of cotyledon development where neither dense vesicles (DVs) nor a PSV were recognizable. However, at later stages of cotyledon development, condensed globulins were visible in both DVs and distinct MVBs with a novel form of partitioning, with the internal vesicles being pushed to one sector of this organelle. These distinct MVBs were no longer sensitive to wortmannin. This study thus indicates a possible role for MVBs in transporting storage proteins to PSVs during the early stage of seed development prior to the involvement of DVs. In addition, wortmannin treatment is shown to induce DVs to form aggregates and to fuse with the plasma membrane.
ISSN:0022-0957
1460-2431
DOI:10.1093/jxb/err366