Activated human CD4+ T cells express transporters for both cysteine and cystine

Because naïve T cells are unable to import cystine due to the absence of cystine transporters, it has been suggested that T cell activation is dependent on cysteine generated by antigen presenting cells. The aim of this study was to determine at which phases during T cell activation exogenous cystin...

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Bibliographic Details
Published in:Scientific reports 2012-02, Vol.2 (1), p.266-266, Article 266
Main Authors: Levring, Trine Bøegh, Hansen, Ann Kathrine, Nielsen, Bodil Lisbeth, Kongsbak, Martin, von Essen, Marina Rode, Woetmann, Anders, Ødum, Niels, Bonefeld, Charlotte Menné, Geisler, Carsten
Format: Article
Language:English
Subjects:
631/136/142
631/250/2502
631/80/641
631/80/86
Antigen-presenting cells
Antigens
CD4 antigen
CD4-Positive T-Lymphocytes - metabolism
Cell growth
Cells, Cultured
Cysteine - metabolism
Cystine - metabolism
Humanities and Social Sciences
Humans
Immunology
Lymphocyte Activation
Lymphocytes
Lymphocytes T
Membrane Transport Proteins - metabolism
multidisciplinary
Polymerase Chain Reaction
Science
T cell receptors
Up-Regulation
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Summary:Because naïve T cells are unable to import cystine due to the absence of cystine transporters, it has been suggested that T cell activation is dependent on cysteine generated by antigen presenting cells. The aim of this study was to determine at which phases during T cell activation exogenous cystine/cysteine is required and how T cells meet this requirement. We found that early activation of T cells is independent of exogenous cystine/cysteine, whereas T cell proliferation is strictly dependent of uptake of exogenous cystine/cysteine. Naïve T cells express no or very low levels of both cystine and cysteine transporters. However, we found that these transporters become strongly up-regulated during T cell activation and provide activated T cells with the required amount of cystine/cysteine needed for T cell proliferation. Thus, T cells are equipped with mechanisms that allow T cell activation and proliferation independently of cysteine generated by antigen presenting cells.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep00266