HBL-1 Patterns Synaptic Remodeling in C. elegans

• Published in: Neuron (Cambridge, Mass.) Mass.), 2012-02, Vol.73 (3), p.453-465
• Main Authors: Thompson-Peer, Katherine L., Bai, Jihong, Hu, Zhitao, Kaplan, Joshua M.
• Format: Article
• Language: English
• Subjects: Animals; Animals, Genetically Modified; Behavior; Behavior, Animal; Body Patterning - genetics; Caenorhabditis elegans - anatomy & histology; Caenorhabditis elegans - physiology; Caenorhabditis elegans Proteins - genetics; Caenorhabditis elegans Proteins - metabolism; Caenorhabditis elegans Proteins - physiology; Circuits; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Embryo, Nonmammalian; Embryos; gamma-Aminobutyric Acid - metabolism; Gene Expression Regulation, Developmental - genetics; Genes; Green Fluorescent Proteins - genetics; Motor Neurons - physiology; Mutation - genetics; Neuromuscular Junction - embryology; Neuromuscular Junction - genetics; Neurons; Receptors, Cell Surface - physiology; Receptors, Cytoplasmic and Nuclear - physiology; Studies; Transcription Factors - genetics; Transcription Factors - metabolism; Vertebrates
• ISSN: 0896-6273; 1097-4199
• DOI: 10.1016/j.neuron.2011.11.025

During development, circuits are refined by the dynamic addition and removal of synapses; however, little is known about the molecular mechanisms that dictate where and when synaptic refinement occurs. Here we describe transcriptional mechanisms that pattern remodeling of C. elegans neuromuscular junctions (NMJs). The embryonic GABAergic DD motor neurons remodel their synapses, whereas the later born VD neurons do not. This specificity is mediated by differential expression of a transcription factor (HBL-1), which is expressed in DD neurons but is repressed in VDs by UNC-55/COUP-TF. DD remodeling is delayed in hbl-1 mutants whereas precocious remodeling is observed in mutants lacking the microRNA mir-84, which inhibits hbl-1 expression. Mutations increasing and decreasing circuit activity cause corresponding changes in hbl-1 expression, and corresponding shifts in the timing of DD plasticity. Thus, convergent regulation of hbl-1 expression defines a genetic mechanism that patterns activity-dependent synaptic remodeling across cell types and across developmental time. ► D neuron remodeling is restricted to DD neurons and to a specific developmental time ► Remodeling of VD neurons is prevented by UNC-55 mediated repression of hbl-1 ► Bidirectional changes in hbl-1 levels shift the timing of DD neuron remodeling ► microRNAs and circuit activity regulate hbl-1 levels to control synaptic remodeling The molecular mechanisms involved in activity-dependent synaptic remodeling are only partially understood. Thompson-Peer describe a new role for transcriptional mechanisms involving the transcription factor HBL-1 and the regulatory miRNA mir-84 in remodeling of synapses at the C. elegans NMJ.