Further studies on partially purified calf thymus DNA polymerase a

Attempts to prevent the urea conversion of a 200–230, 000 molecular weight DNA polymerase a to a 150–170,000 molecular weight form by the inclusion of protease inhibitors have not been successful. No other method has been found capable of dissociating a 50–70,000 fragment or subunit from the DNA pol...

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Bibliographic Details
Published in:Nucleic acids research 1979-07, Vol.6 (10), p.3341-3352
Main Authors: McKune, K, Holmes, A.M
Format: Article
Language:English
Subjects:
animal science
Animals
Cattle
DNA Polymerase II - isolation & purification
DNA Polymerase II - metabolism
DNA-Directed DNA Polymerase - metabolism
Kinetics
livestock
Molecular Weight
Structure-Activity Relationship
Templates, Genetic
Thymus Gland - enzymology
zoology
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Summary:Attempts to prevent the urea conversion of a 200–230, 000 molecular weight DNA polymerase a to a 150–170,000 molecular weight form by the inclusion of protease inhibitors have not been successful. No other method has been found capable of dissociating a 50–70,000 fragment or subunit from the DNA polymerase subunit. Addition of this 50–70,000 subunit to the polymerase subunit does not aid the binding of the enzyme to DNA, but does have an effect on the utilisation of synthetic template-initiator complexes by the polymerase subunit.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/6.10.3341