Phylogenetic analysis of serine proteases from Russell’s viper (Daboia russelli siamensis) and Agkistrodon piscivorus leucostoma venom

• Published in: Toxicon (Oxford) 2011-08, Vol.58 (2), p.168-178
• Main Authors: Sukkapan, Pattadon, Jia, Ying, Nuchprayoon, Issarang, Pérez, John C.
• Format: Article
• Language: English
• Subjects: 3' Untranslated Regions; Agkistrodon - metabolism; Agkistrodon piscivorus; Agkistrodon piscivorus leucostoma; Algorithms; Amino Acid Sequence; Animal poisons toxicology. Antivenoms; Animals; Base Sequence; Beta; Biological and medical sciences; cDNA; cDNA libraries; Coding; complementary DNA; Crotalid Venoms - enzymology; Daboia - metabolism; Databases, Nucleic Acid; Evolution; Gene Library; hemostasis; Leucostoma; Libraries; Medical sciences; Molecular Sequence Data; open reading frames; Phylogeny; plasminogen activator; Protease; Proteins; Reptilian Proteins - chemistry; Reptilian Proteins - genetics; Reverse Transcriptase Polymerase Chain Reaction; RT-PCR; Russell’s viper; Sequence Alignment; Sequence Analysis, DNA; Serine Endopeptidases - chemistry; Serine Endopeptidases - genetics; Snake venom serine protease; Snakes; thrombin; Toxicology; Trees; venoms; Viper Venoms - enzymology
• ISSN: 0041-0101; 1879-3150
• DOI: 10.1016/j.toxicon.2011.05.014

Serine proteases are widely found in snake venoms. They have variety of functions including contributions to hemostasis. In this study, five serine proteases were cloned and characterized from two different cDNA libraries: factor V activator (RVV-V), alpha fibrinogenase (RVAF) and beta fibrinogenase (RVBF) from Russell’s viper (Daboia russelli siamensis), and plasminogen activator (APL-PA) and protein C activator (APL-C) from Agkistrodon piscivorus leucostoma. The snake venom serine proteases were clustered in phylogenetic tree according to their functions. KA/KS values suggested that accelerated evolution has occurred in the mature protein coding regions in cDNAs of snake venom serine proteases. ► cDNAs of venom serine proteases from D. r. siamensis and A. p. leucostoma were cloned. ► The amino acid sequences of the cDNAs were analyzed by multiple sequence alignment. ► Phylogenetic trees were constructed to study their evolutionary relationship. ► KA/KS values suggested that accelerated evolution has occurred.