Use of interspecies hybrids to clone mutant alleles in Drosophila

To increase the number of useful RFLPs, we have taken advantage of the ability of Drosophila melanogaster to form viable interspecific hybrids with Drosophila simulans. In mice, the use of interspecific backcrosses has greatly facilitated genetic mapping, because laboratory strains of Mus musculus a...

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Bibliographic Details
Published in:Nucleic acids research 1994-10, Vol.22 (20), p.4346-4347
Main Authors: Billin, A.N, Poole, S.J
Format: Article
Language:English
Subjects:
Alleles
Animals
Blotting, Southern
cloning
Cloning, Molecular
Drosophila melanogaster
Drosophila melanogaster - genetics
Drosophila simulans
Hybridization, Genetic
interspecific hybridization
mutants
Mutation
Polymorphism, Restriction Fragment Length
restriction fragment length polymorphism
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Summary:To increase the number of useful RFLPs, we have taken advantage of the ability of Drosophila melanogaster to form viable interspecific hybrids with Drosophila simulans. In mice, the use of interspecific backcrosses has greatly facilitated genetic mapping, because laboratory strains of Mus musculus and the closely related species Mus spretus are sufficiently divergent that most cloned probes can detect RFLPs between the two species. Crossing the two species produces fertile hybrid female offspring heterozygous for RFLPs at most loci. Interspecific hybrids between D. melanogaster and D. simulans could also contain numerous RFLPs potentially useful for the identification of alleles arising from particular D. melanogaster chromosomes.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/22.20.4346