A PPARγ–FGF1 axis is required for adaptive adipose remodelling and metabolic homeostasis

• Published in: Nature (London) 2012-05, Vol.485 (7398), p.391-394
• Main Authors: Jonker, Johan W., Suh, Jae Myoung, Atkins, Annette R., Ahmadian, Maryam, Li, Pingping, Whyte, Jamie, He, Mingxiao, Juguilon, Henry, Yin, Yun-Qiang, Phillips, Colin T., Yu, Ruth T., Olefsky, Jerrold M., Henry, Robert R., Downes, Michael, Evans, Ronald M.
• Format: Article
• Language: English
• Subjects: 631/337; 631/443/319/1557; 631/45/287/1182; 631/45/612/388; Adipocytes - drug effects; Adipocytes - metabolism; Adipocytes - pathology; Adipose tissues; Animals; Base Sequence; Cell Size - drug effects; Diabetes Mellitus, Experimental - chemically induced; Diabetes Mellitus, Experimental - genetics; Diabetes Mellitus, Experimental - pathology; Diet, High-Fat - adverse effects; Fibroblast Growth Factor 1 - deficiency; Fibroblast Growth Factor 1 - genetics; Fibroblast Growth Factor 1 - metabolism; Fibroblast growth factors; Genes; Genetic aspects; Homeostasis - drug effects; Humanities and Social Sciences; Humans; Inflammation - genetics; Insulin - metabolism; Insulin Resistance; Intra-Abdominal Fat - drug effects; Intra-Abdominal Fat - metabolism; Intra-Abdominal Fat - pathology; letter; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; multidisciplinary; Necrosis - enzymology; Physiological aspects; PPAR gamma - metabolism; Promoter Regions, Genetic - genetics; Response Elements - genetics; Science; Science (multidisciplinary)
• ISSN: 0028-0836; 1476-4687
• DOI: 10.1038/nature10998

PPARγ induces fibroblast growth factor 1 to remodel visceral adipose tissue in response to a high-fat diet to maintain metabolic homeostasis. Growth factor's role in homeostasis Fibroblast growth factor 1 (FGF1) and the other known FGF family proteins have been implicated in a range of physiological processes but, surprisingly, FGF1 knockout mice display no obvious abnormality. Here the authors show that FGF1 is induced in visceral adipose tissue in response to a high-fat diet. This induction of FGF1 in the fed state is regulated by the nuclear receptor PPARγ. Mice without FGF1 have an impaired metabolic response of visceral adipose in response to high-fat diet, and develop an aggressive diabetic phenotype coupled to aberrant adipose expansion. The discovery of a phenotype for the FGF1 knockout mouse establishes the PPARγ–FGF1 axis as crucial for maintaining metabolic homeostasis and insulin sensitization. Although feast and famine cycles illustrate that remodelling of adipose tissue in response to fluctuations in nutrient availability is essential for maintaining metabolic homeostasis, the underlying mechanisms remain poorly understood 1 , 2 . Here we identify fibroblast growth factor 1 (FGF1) as a critical transducer in this process in mice, and link its regulation to the nuclear receptor PPARγ (peroxisome proliferator activated receptor γ), which is the adipocyte master regulator and the target of the thiazolidinedione class of insulin sensitizing drugs 3 , 4 , 5 . FGF1 is the prototype of the 22-member FGF family of proteins and has been implicated in a range of physiological processes, including development, wound healing and cardiovascular changes 6 . Surprisingly, FGF1 knockout mice display no significant phenotype under standard laboratory conditions 7 , 8 , 9 . We show that FGF1 is highly induced in adipose tissue in response to a high-fat diet and that mice lacking FGF1 develop an aggressive diabetic phenotype coupled to aberrant adipose expansion when challenged with a high-fat diet. Further analysis of adipose depots in FGF1-deficient mice revealed multiple histopathologies in the vasculature network, an accentuated inflammatory response, aberrant adipocyte size distribution and ectopic expression of pancreatic lipases. On withdrawal of the high-fat diet, this inflamed adipose tissue fails to properly resolve, resulting in extensive fat necrosis. In terms of mechanisms, we show that adipose induction of FGF1 in the fed state is regulated by PPA