The complete coding sequence of the human A-raf-1 oncogene and transforming activity of a human A-raf carrying retrovirus

The complete 606 amino acid sequence of the human A-raf oncogene has been deduced from the 2453 nucleotide sequence of a human T cell cDNA. A cysteine-rich region located near the amino terminus, which is highly conserved in A-raf and c-raf, shows significant homology with protein kinase C. A 5'...

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Bibliographic Details
Published in:Nucleic acids research 1987-01, Vol.15 (2), p.595-609
Main Authors: BECK, T. W, HULEIHEL, M, GUNNELL, M, BONNER, T. I, RAPP, U. R
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Applied sciences
Base Sequence
cDNA
Cell Transformation, Neoplastic
DNA - analysis
Exact sciences and technology
genes
Genes, Viral
Humans
Liver - embryology
Mice
Moloney murine sarcoma virus - genetics
nucleotide sequence
Oncogenes
Other techniques and industries
Sarcoma Viruses, Murine - genetics
T-Lymphocytes - analysis
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Summary:The complete 606 amino acid sequence of the human A-raf oncogene has been deduced from the 2453 nucleotide sequence of a human T cell cDNA. A cysteine-rich region located near the amino terminus, which is highly conserved in A-raf and c-raf, shows significant homology with protein kinase C. A 5' deleted fragment of the cDNA has been incorporated into a murine retrovirus which endows the virus with the ability to transform cells in vivo and in vitro. Functionally, human A-raf is similar to v-raf and v-mos in that transformation is independent of ras gene function.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/15.2.595