Nucleosome dissociation and transfer in concentrated salt solutions

We have examined the dissociation of nucleosomes into histones and free, 4.5S DNA over a range of sodium chloride concentrations between 0.25 and 1 M. We have also studied this dissociation as a function of nucleosome concentration at two salt concentrations, 0.8 M and 0.9 M. In addition, we have me...

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Bibliographic Details
Published in:Nucleic acids research 1979-12, Vol.7 (8), p.2457-2467
Main Authors: Stacks, Pamela C., Schumaker, Verne N.
Format: Article
Language:English
Subjects:
Animals
Chickens
DNA - analysis
DNA, Viral - isolation & purification
Erythrocytes - analysis
Histones - analysis
Kinetics
Nucleosomes - analysis
Osmolar Concentration
Protein Binding
T-Phages - analysis
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Summary:We have examined the dissociation of nucleosomes into histones and free, 4.5S DNA over a range of sodium chloride concentrations between 0.25 and 1 M. We have also studied this dissociation as a function of nucleosome concentration at two salt concentrations, 0.8 M and 0.9 M. In addition, we have measured the kinetics of transfer of histone cores from nucleosomes onto recipient bacteriophage T7 DNA in 0.6, 0.7 and 0.8 M NaCl solutions. Although the mechanism of nucleosome transfer is unknown the data presented here are consistent with either a reversible dissociation of the nucleosome or DNA strand displacement by another DNA.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/7.8.2457