An NMR study of the exchange rates for protons involved in the secondary and tertiary structure of yeast tRNA Phe

Solvent exchange rates of all the protons of yeast tRNAphe resonating in the lowfield NMR region (-11 to-15 ppm from DSS) have been measured by saturation-recovery long-pulse Fourier transform NMR. All these protons in yeast tRNAphe are in the fast exchange limit with H2O relative to their intrinsic...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic acids research 1977-10, Vol.4 (10), p.3599-3615
Main Authors: Johnston, P D, Redfield, A G
Format: Article
Language:English
Subjects:
Anticodon
Hydrogen Bonding
Kinetics
Magnesium
Magnetic Resonance Spectroscopy
Models, Molecular
Nucleic Acid Conformation
Nucleic Acid Denaturation
Phenylalanine
RNA, Transfer
Saccharomyces cerevisiae
Temperature
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Solvent exchange rates of all the protons of yeast tRNAphe resonating in the lowfield NMR region (-11 to-15 ppm from DSS) have been measured by saturation-recovery long-pulse Fourier transform NMR. All these protons in yeast tRNAphe are in the fast exchange limit with H2O relative to their intrinsic longitudinal relaxation processes. Most rates show very little temperature dependence; however, tertiary base pair protons are preferentially destabilized in the absence of Mg++ at higher temperatures. The measured exchange rates are between 2 and 125 sec-1 for a temperature range from 10 degrees C to 45 degrees C and MgCl2 concentrations between 0 and 15 mM.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/4.10.3599