Design and application of genetically encoded biosensors

In the past 5–10 years, the power of the green fluorescent protein (GFP) and its numerous derivatives has been harnessed toward the development of genetically encoded fluorescent biosensors. These sensors are incorporated into cells or organisms as plasmid DNA, which leads the transcriptional and tr...

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Bibliographic Details
Published in:Trends in biotechnology (Regular ed.) 2011-03, Vol.29 (3), p.144-152
Main Authors: Palmer, Amy E, Qin, Yan, Park, Jungwon Genevieve, McCombs, Janet E
Format: Article
Language:English
Subjects:
Animals
Biochemistry
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biosensors
Biotechnology
Derivatives
Enzymes
Fluorescent Dyes - chemistry
Fluorescent Dyes - metabolism
Fundamental and applied biological sciences. Psychology
Green Fluorescent Proteins - chemistry
Green Fluorescent Proteins - genetics
Humans
Internal Medicine
Kinases
Methods. Procedures. Technologies
Organisms
Peptides
Proteins
Real time
Sensors
Various methods and equipments
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Summary:In the past 5–10 years, the power of the green fluorescent protein (GFP) and its numerous derivatives has been harnessed toward the development of genetically encoded fluorescent biosensors. These sensors are incorporated into cells or organisms as plasmid DNA, which leads the transcriptional and translational machinery of the cell to express a functional sensor. To date, over 100 different genetically encoded biosensors have been developed for targets as diverse as ions, molecules and enzymes. Such sensors are instrumental in providing a window into the real-time biochemistry of living cells and whole organisms, and are providing unprecedented insight into the inner workings of a cell.
ISSN:0167-7799
1879-3096
DOI:10.1016/j.tibtech.2010.12.004