In vivo recombination of cauliflower mosaic virus DNA

Ligation and recombination of the DNA of cauliflower mosaic virus (CaMV) is demonstrated by the following experiments: (i) Ligation: Different noninfectious fragments of the CaMV genome (obtained after insertion into plasmid pBR322 followed by enzymatic excision) regained infectivity when mixtures o...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1982-05, Vol.79 (9), p.2932-2936
Main Authors: Lebeurier, G, Hirth, L, Hohn, B, Hohn, T
Format: Article
Language:English
Subjects:
Barns
Biological Sciences: Cell Biology
cauliflower mosaic virus
DNA
Genes
Genomes
Hybridity
Leaves
Ligation
Plasmids
Viral DNA
Virions
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Summary:Ligation and recombination of the DNA of cauliflower mosaic virus (CaMV) is demonstrated by the following experiments: (i) Ligation: Different noninfectious fragments of the CaMV genome (obtained after insertion into plasmid pBR322 followed by enzymatic excision) regained infectivity when mixtures of them were used to inoculate their host. The symptom appearance was delayed by comparison with a typical CaMV infection, and only the newly formed leaves were affected. (ii) Recombination: Pairs of noninfectious recombinant full-length CaMV genomes (integrated into pBR322 at different restriction endonuclease sites) regained infectivity upon simultaneous inoculation of a sensitive host. The symptomatology of the resulting infection was indistinguishable from that of a typical CaMV infection. We show that progeny DNA had the same characteristics (size, structure, restriction endonuclease digestion pattern) as bona fide CaMV DNA, and that the vector pBR322 had been completely eliminated. A cloned tandem dimer of CaMV DNA with a partial deletion similarly was infectious in the plant assays. This system should be useful to study the expression of mutant genomes, thus allowing characterization of the CaMV genes.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.79.9.2932