Cell sheet technology for regeneration of esophageal mucosa

• Published in: World journal of gastroenterology : WJG 2012-10, Vol.18 (37), p.5145-5150
• Main Authors: Takagi, Ryo, Yamato, Masayuki, Kanai, Nobuo, Murakami, Daisuke, Kondo, Makoto, Ishii, Takaaki, Ohki, Takeshi, Namiki, Hideo, Yamamoto, Masakazu, Okano, Teruo
• Format: Article
• Language: English
• Subjects: Animals; Cell Transplantation - methods; Cells, Cultured - cytology; Dogs; Epithelial Cells - cytology; Esophagus - cytology; Esophagus - physiology; Humans; Inflammation; Mice; Mouth Mucosa - cytology; Mouth Mucosa - physiology; Mouth Mucosa - transplantation; Regeneration; Regenerative Medicine - instrumentation; Regenerative Medicine - methods; Swine; Tissue Engineering - instrumentation; Tissue Engineering - methods; Tissue Scaffolds - chemistry; 上皮细胞; 临床应用; 再生医学; 再生技术; 口腔黏膜; 电池板; 胃肠道肿瘤; 食管癌
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v18.i37.5145

The progress of tissue-engineering technology has realized development of new therapies to treat vari- ous disorders by using cultured cells. Cell- and tissue- based therapies have been successfully applied to hu- man patients, and several tissue-engineered products have been approved by the regulatory agencies and are commercially available. In the review article, we describe our experience of development and clinical application of cell sheet-based regenerative medicine.Endoscopic mucosal resection （EMR） and endoscopic submucosal dissection （ESD） have been shown to be useful for removal of gastrointestinal neoplasms with less invasiveness compared with open surgery, espe- cially in esophageal surgery. However, postoperative inflammation and stenosis are major complications observed after intensive mucosal resection. Therefore, we have developed novel regenerative medicine to pre- vent such complications and promote wound healing of esophageal mucosa after EMR or ESD. Transplantable oral mucosal epithelial cell sheets were fabricated from patients＇ own oral mucosa. Immediately after EMR or ESD, fabricated autologous cell sheets were endoscopi- cally transplanted to the ulcer sites. We performed a preclinical study with a canine model. In human clini- cal settings, cell culture and cell sheet fabrication were performed in clean rooms according to good manufac- turing practice guidelines, and pharmaceutical drugs were used as supplements to culture medium in place of research regents used in animal study. We believe that cell-based regenerative medicine would be useful to improve quality of life of patients after EMR or ESD.