Lucifer Yellow uptake by CHO cells exposed to magnetic and electric pulses

• Published in: Radiology and oncology 2012-06, Vol.46 (2), p.119-125
• Main Authors: Towhidi, Leila, Firoozabadi, Seyed, Mozdarani, Hossein, Miklavcic, Damijan
• Format: Article
• Language: English
• Subjects: Biophysics; Electric fields; electro-endocytosis; electroporation; Magnetic fields; Membranes; Molecular biology; time-varying magnetic field; transmembrane molecular transport
• ISSN: 1318-2099; 1581-3207; 0485-893X
• DOI: 10.2478/v10019-012-0014-2

The cell membrane acts as a barrier that hinders free entrance of most hydrophilic molecules into the cell. Due to numerous applications in medicine, biology and biotechnology, the introduction of impermeant molecules into biological cells has drawn considerable attention in the past years. One of the most famous methods in this field is electroporation, in which electric pulses with high intensity and short duration are applied to the cells. The aim of our study was to investigate the effect of time-varying magnetic field with different parameters on transmembrane molecular transport. 'Moreover, a comparison was made between the uptake results due to magnetic pulse exposure and electroporation mediated uptake.' at the end of Background part. The Chinese hamster ovary (CHO) cells were exposed to magnetic pulses of 2.2 T peak strength and 250 μs duration delivered by Magstim stimulator and double 70 mm coil. Three different frequencies of 0.25, 1 and 10 Hz pulses with 112, 56 and 28 number of pulses were applied (altogether nine experimental groups) and Lucifer Yellow uptake was measured in each group. Moreover, maximum uptake of Lucifer Yellow obtained by magnetic pulses was compared to the measured uptake due to electroporation with typical parameters of 8 pulses of 100 μs, repetition frequency of 1 Hz and electric field intensities of 200 to 600 V/cm. Our results show that time-varying magnetic field exposure increases transmembrane molecular transport and this uptake is greater for lower frequencies and larger number of pulses. Besides, the comparison shows that electroporation is more effective than pulsed magnetic field, but the observed uptake enhancement due to magnetic exposure is still considerable.