Gemi : PCR Primers Prediction from Multiple Alignments

Designing primers and probes for polymerase chain reaction (PCR) is a preliminary and critical step that requires the identification of highly conserved regions in a given set of sequences. This task can be challenging if the targeted sequences display a high level of diversity, as frequently encoun...

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Bibliographic Details
Published in:Comparative and functional genomics 2012-01, Vol.2012 (2012), p.1-5
Main Authors: Sobhy, Haitham, Colson, Philippe
Format: Article
Language:English
Subjects:
Bioinformatics
DNA probes
Polymerase chain reaction
Primers
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Summary:Designing primers and probes for polymerase chain reaction (PCR) is a preliminary and critical step that requires the identification of highly conserved regions in a given set of sequences. This task can be challenging if the targeted sequences display a high level of diversity, as frequently encountered in microbiologic studies. We developed Gemi, an automated, fast, and easy-to-use bioinformatics tool with a user-friendly interface to design primers and probes based on multiple aligned sequences. This tool can be used for the purpose of real-time and conventional PCR and can deal efficiently with large sets of sequences of a large size.
ISSN:1531-6912
2314-436X
1532-6268
2314-4378
DOI:10.1155/2012/783138