Effect of Electroacupuncture Intervention on Expression of CGRP, SP, COX-1, and PGE2 of Dorsal Portion of the Cervical Spinal Cord in Rats with Neck-Incision Pain

The present study was aimed to determine if cervicospinal substance P (SP) and its neurokinin-1 receptor (NK-1R), calcitonin gene-related peptide (CGRP), cyclooxygenase-1 (COX-1), and prostaglandin E2 (PGE2) were involved in electroacupuncture (EA) analgesia in neck-incision pain rats. EA interventi...

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Bibliographic Details
Published in:Evidence-based complementary and alternative medicine 2013-01, Vol.2013 (2013), p.1-9
Main Authors: Wang, Jun-ying, Chen, Shu-ping, Qiao, Li-na, Liu, Jun-ling, Zhang, Jian-liang, Gao, Yong-hui, Yang, Yong-sheng
Format: Article
Language:English
Subjects:
Acupuncture
Analgesia
Analgesics
Calcitonin
Calcitonin gene-related peptide
Chinese medicine
Cyclooxygenase-1
Electroacupuncture
Enzyme-linked immunosorbent assay
Gene expression
Immunofluorescence
Laboratory animals
Neck
Neurokinin
Neurokinin NK1 receptors
Neurosciences
Pain
Pain perception
Prostaglandin E2
Rats
Rodents
Spinal cord
Stimulation
Substance P
Surgery
Thyroid
Thyroid gland
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Summary:The present study was aimed to determine if cervicospinal substance P (SP) and its neurokinin-1 receptor (NK-1R), calcitonin gene-related peptide (CGRP), cyclooxygenase-1 (COX-1), and prostaglandin E2 (PGE2) were involved in electroacupuncture (EA) analgesia in neck-incision pain rats. EA intervention was applied to bilateral Futu (LI18), Hegu (LI4)-Neiguan (PC6), and Zusanli (ST36)-Yanglingquan (GB34) for 30 min. Cervicospinal SP and CGRP immunoactivity was detected by immunofluorescence technique, NK-1R and COX-1 protein and mRNA expression levels were determined using Western blot and real-time PCR, respectively, and PGE2 content was measured using ELISA. Outcomes indicated that EA of EA-LI18 and LI4-PC6 (not ST36-GB34) significantly suppressed neck-incision induced decrease of thermal pain threshold (P
ISSN:1741-427X
1741-4288
DOI:10.1155/2013/294091