Efficient genome editing in plants using a CRISPR/Cas system

• Published in: Cell research 2013-10, Vol.23 (10), p.1229-1232
• Main Authors: Feng, Zhengyan, Zhang, Botao, Ding, Wona, Liu, Xiaodong, Yang, Dong-Lei, Wei, Pengliang, Cao, Fengqiu, Zhu, Shihua, Zhang, Feng, Mao, Yanfei, Zhu, Jian-Kang
• Format: Article
• Language: English
• Subjects: 631/1647/1511; 631/449/447; Arabidopsis - genetics; Biomedical and Life Sciences; CAS系统; Cell Biology; Clustered Regularly Interspaced Short Palindromic Repeats; CRISPR-Associated Proteins - genetics; DNA双链断裂; DNA核酸; DNA结合结构域; Gene Targeting; Genome, Plant; Letter to the Editor; Life Sciences; Mutation; Oryza - genetics; Plant Proteins - genetics; 基因组; 植物; 编辑; 非编码RNA
• ISSN: 1001-0602; 1748-7838
• DOI: 10.1038/cr.2013.114

Dear Editor, In the past few years, the development of sequence- specific DNA nucleases has progressed rapidly and such nucleases have shown their power in generating efficient targeted mutagenesis and other genome editing applica- tions. For zinc finger nucleases （ZFNs） and transcription activator-like effector nucleases （TALENs）, an engi- neered array of sequence-specific DNA binding domains are fused with the DNA nuclease Fokl [1, 2]. These nu- cleases have been successful in genome modifications by generating double strand breaks （DSBs）, which are then repaired through non-homologous end joining （NHEJ） or homologous recombination （HR） in different species, including mouse, tobacco and rice [3-5]. Recently, an- other breakthrough technology for genome editing, the CRISPR/Cas system, was developed. CRISPR （clustered regulatory interspaced short 12alindromic repeats） loci are variable short spacers separated by short repeats, which are transcribed into non-coding RNAs. The non-coding RNAs form a functional complex with CRISPR-asso- ciated （Cas） proteins and guide the complex to cleave complementary invading DNA [6]. After the initial development of a programmable CRISPR/Cas system, it has been rapidly applied to achieve efficient genome editing in human cell lines, zebrafish and mouse [7-10]. However, there is still no successful application in plants reported.