Pectinesterase Inhibitor from Jelly Fig (Ficus awkeotsang Makino) Achene Inhibits Surface Antigen Expression by Human Hepatitis B Virus

Pectinesterase inhibitor (PEI) isolated from jelly fig (Ficus awkeotsang Makino) is an edible component of a popular drink consumed in Asia. Hepatitis B virus (HBV) infection is prevalent in Asia, and current treatments for HBV infection need improvement. This study aimed to evaluate the effect of P...

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Bibliographic Details
Published in:Evidence-based complementary and alternative medicine 2013-01, Vol.2013 (2013), p.1-5
Main Authors: Huang, Yu-Chuen, Jiang, Chii-Ming, Chen, Yu-Jen, Chen, Yu-Yawn
Format: Article
Language:English
Subjects:
Animal models
Antigens
Apoptosis
Cell culture
Cell cycle
Cell lines
Chemotherapy
Colorimetry
Cytotoxicity
Enzyme-linked immunosorbent assay
Ficus
Ficus pumila awkeotsang
Gene expression
Genomes
Hepatitis
Hepatitis B
Hepatitis B surface antigen
Hepatitis B virus
Hepatocytes
Hepatology
Hepatoma
Infections
Liver cancer
Medical prognosis
Mutation
Pectinesterase
Virology
Viruses
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Summary:Pectinesterase inhibitor (PEI) isolated from jelly fig (Ficus awkeotsang Makino) is an edible component of a popular drink consumed in Asia. Hepatitis B virus (HBV) infection is prevalent in Asia, and current treatments for HBV infection need improvement. This study aimed to evaluate the effect of PEI on the surface antigen expression by HBV (HBsAg). Human hepatoma cell lines Hep3B and Huh7 served as in vitro models for assessing the cytotoxicity and HBsAg expression. A culture of primary hepatocytes cultured from mice served as the normal counterpart. Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. HBsAg expression was evaluated by measuring HBsAg secretion into the culture medium using an enzyme-linked immunosorbent assay. The results showed that PEI did not affect the viability of the human hepatoma cell lines or primary mouse hepatocytes. PEI inhibited the expression of HBsAg in hepatoma cell lines harboring endogenous (Hep3B) and integrated (Huh7) HBV genomes in a concentration- and time-dependent manner, thus implicating a universal activity against HBV gene expression. In conclusion, it suggests that PEI from jelly fig inhibits the expression of human HBsAg in host cells without toxic effects on normal primary hepatocytes.
ISSN:1741-427X
1741-4288
DOI:10.1155/2013/434823