Molecular Cloning and Primary Nucleotide Sequence Analysis of a Distinct Human Immunodeficiency Virus Isolate Reveal Significant Divergence in Its Genomic Sequences

In an effort to evaluate data on genomic relatedness among the various human immunodeficiency viruses (HIVs), we have molecularly cloned a virus isolate designated HIV (CDC-451). Preliminary characterization of the HIV (CDC-451) clone indicated that the restriction enzyme map was distinct from those...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1986-11, Vol.83 (21), p.8380-8384
Main Authors: Desai, S. M., Kalyanaraman, V. S., Casey, J. M., Srinivasan, A., Andersen, P. R., Devare, S. G.
Format: Article
Language:English
Subjects:
Adolescent
AIDS
AIDS/HIV
Amino Acid Sequence
Amino acids
Base Sequence
Biological and medical sciences
Cloning, Molecular
DNA
Fundamental and applied biological sciences. Psychology
gag genes
Gene Products, gag
Genes, Regulator
Genes, Viral
Genetics
Glycoproteins
Glycoproteins - analysis
HIV
HIV - genetics
Humans
Male
Microbiology
Nucleotide sequences
Open reading frames
Retroviridae
Retroviridae Proteins - genetics
Viral Envelope Proteins - analysis
Viral Envelope Proteins - genetics
Virology
Viruses
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Summary:In an effort to evaluate data on genomic relatedness among the various human immunodeficiency viruses (HIVs), we have molecularly cloned a virus isolate designated HIV (CDC-451). Preliminary characterization of the HIV (CDC-451) clone indicated that the restriction enzyme map was distinct from those of other known HIV isolates. Analysis of the primary nucleotide sequence of the regions encoding the structural proteins and comparison with sequences known for other HIV isolates indicated substantial differences for HIV (CDC-451). The sequences encoding the group-specific antigen gene, although they showed some variation, were conserved to a greater extent than were those encoding envelope proteins. In the envelope gene sequences, most of the changes (up to 24.5% divergence) were located in the amino-terminal region encoding a glycoprotein with a Mrof 120,000. The carboxyl-terminal region, encoding a protein of Mr41,000, was more highly conserved. The variation in the sequences encoding envelope proteins may have important implications for the antigenic properties and/or pathogenicity of the disease and for its detection and ultimate eradication.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.83.21.8380