Assignment of the Gene for Human DNA Polymerase α to the X Chromosome

We have applied an assay based on a monoclonal antibody that discriminates the activity of human DNA polymerase α in rodent--human somatic cell hybrid clones to identify a single genetic locus that is both necessary and sufficient for the expression of DNA polymerase α . We have mapped this locus to...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1985-08, Vol.82 (16), p.5270-5274
Main Authors: Wang, Teresa Shu-Fong, Pearson, Barbara E., Suomalainen, Heli A., Mohandas, T., Shapiro, Larry J., Schröder, Jim, Korn, David
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Antibodies, Monoclonal
Biological and medical sciences
Cell Line
Cell lines
Chromosome Mapping
Chromosomes
Clone Cells
DNA
DNA Polymerase II - genetics
Epitopes - analysis
Female
Fundamental and applied biological sciences. Psychology
Genes
Genetic loci
Human X chromosome
Humans
Hybrid Cells - enzymology
Hybridity
Hypoxanthine Phosphoribosyltransferase - deficiency
KB cells
Lymphocytes - enzymology
Mice
Monoclonal antibodies
Nucleic acids
Nucleic bases, nucleotides
Species Specificity
X Chromosome
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Summary:We have applied an assay based on a monoclonal antibody that discriminates the activity of human DNA polymerase α in rodent--human somatic cell hybrid clones to identify a single genetic locus that is both necessary and sufficient for the expression of DNA polymerase α . We have mapped this locus to the short arm of the human X chromosome, near the junction of bands Xp21.3 and Xp22.1, and demonstrated that it is not expressed from an inactive X chromosome.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.82.16.5270