Expression profiling of Aldh1l1-precursors in the developing spinal cord reveals glial lineage-specific genes and direct Sox9-Nfe2l1 interactions

Developmental regulation of gliogenesis in the mammalian CNS is incompletely understood, in part due to a limited repertoire of lineage‐specific genes. We used Aldh1l1‐GFP as a marker for gliogenic radial glia and later‐stage precursors of developing astrocytes and performed gene expression profilin...

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Bibliographic Details
Published in:Glia 2013-09, Vol.61 (9), p.1518-1532
Main Authors: Molofsky, Anna V., Glasgow, Stacey M., Chaboub, Lesley S., Tsai, Hui-Hsin, Murnen, Alice T., Kelley, Kevin W., Fancy, Stephen P.J., Yuen, Tracy J., Madireddy, Lohith, Baranzini, Sergio, Deneen, Benjamin, Rowitch, David H., Oldham, Michael C.
Format: Article
Language:English
Subjects:
Age Factors
Aldehyde Dehydrogenase 1 Family
Animals
Astrocytes
Cell Differentiation
Cells, Cultured
Chickens
Computational Biology
Electroporation
Embryo, Mammalian
expression profiling
Flow Cytometry
gene coexpression
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Developmental - physiology
Glial Fibrillary Acidic Protein
gliogenesis
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Isoenzymes - genetics
Isoenzymes - metabolism
Mice
Mice, Transgenic
Nerve Tissue Proteins - genetics
Nerve Tissue Proteins - metabolism
Neuroglia - metabolism
Neurons - classification
Neurons - metabolism
NF-E2-Related Factor 1 - genetics
NF-E2-Related Factor 1 - metabolism
Nfe2l1
Oligonucleotide Array Sequence Analysis
Proteins
Retinal Dehydrogenase - genetics
Retinal Dehydrogenase - metabolism
SOX9 Transcription Factor - genetics
SOX9 Transcription Factor - metabolism
Spinal Cord - cytology
Spinal Cord - embryology
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:Developmental regulation of gliogenesis in the mammalian CNS is incompletely understood, in part due to a limited repertoire of lineage‐specific genes. We used Aldh1l1‐GFP as a marker for gliogenic radial glia and later‐stage precursors of developing astrocytes and performed gene expression profiling of these cells. We then used this dataset to identify candidate transcription factors that may serve as glial markers or regulators of glial fate. Our analysis generated a database of developmental stage‐related markers of Aldh1l1+ cells between murine embryonic day 13.5–18.5. Using these data we identify the bZIP transcription factor Nfe2l1 and demonstrate that it promotes glial fate under direct Sox9 regulatory control. Thus, this dataset represents a resource for identifying novel regulators of glial development.
ISSN:0894-1491
1098-1136
DOI:10.1002/glia.22538