Nonautonomous contact guidance signaling during collective cell migration

• Published in: Proceedings of the National Academy of Sciences - PNAS 2014-02, Vol.111 (5), p.1807-1812
• Main Authors: Londono, Camila, Loureiro, M. Jimena, Slater, Benjamin, Lücker, Petra B., Soleas, John, Sathananthan, Suthamathy, Aitchison, J. Stewart, Kabla, Alexandra J., McGuigan, Alison P.
• Format: Article
• Language: English
• Subjects: Actins; Biological Sciences; Cell Communication; Cell Count; Cell Line; Cell Movement; Cells; Correlations; Density; Epithelial Cells - cytology; Epithelial Cells - metabolism; Fibroblasts; Fibroblasts - cytology; Fibroblasts - metabolism; Humans; Intercellular junctions; Intercellular Junctions - metabolism; Male; Microfilaments; Models, Biological; Morphogenesis; Physical Sciences; Propagation; Signal Transduction; Signals; Speed; Streaming; Velocity
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.1321852111

Directed migration of groups of cells is a critical aspect of tissue morphogenesis that ensures proper tissue organization and, consequently, function. Cells moving in groups, unlike single cells, must coordinate their migratory behavior to maintain tissue integrity. During directed migration, cells are guided by a combination of mechanical and chemical cues presented by neighboring cells and the surrounding extracellular matrix. One important class of signals that guide cell migration includes topographic cues. Although the contact guidance response of individual cells to topographic cues has been extensively characterized, little is known about the response of groups of cells to topographic cues, the impact of such cues on cell–cell coordination within groups, and the transmission of nonautonomous contact guidance information between neighboring cells. Here, we explore these phenomena by quantifying the migratory response of confluent monolayers of epithelial and fibroblast cells to contact guidance cues provided by grooved topography. We show that, in both sparse clusters and confluent sheets, individual cells are contact-guided by grooves and show more coordinated behavior on grooved versus flat substrates. Furthermore, we demonstrate both in vitro and in silico that the guidance signal provided by a groove can propagate between neighboring cells in a confluent monolayer, and that the distance over which signal propagation occurs is not significantly influenced by the strength of cell–cell junctions but is an emergent property, similar to cellular streaming, triggered by mechanical exclusion interactions within the collective system.