Effects of formalin fixation on magnetic resonance indices in multiple sclerosis cortical gray matter

Purpose To investigate changes in magnetic resonance imaging (MRI) indices following formalin fixation of postmortem multiple sclerosis (MS) cortical gray matter (CGM). Postmortem MS brain is being used to establish pathological correlates of changes detected using MRI, with recent emphasis on CGM....

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Bibliographic Details
Published in:Journal of magnetic resonance imaging 2010-11, Vol.32 (5), p.1054-1060
Main Authors: Schmierer, Klaus, Thavarajah, Janet R., An, Shu F., Brandner, Sebastian, Miller, David H., Tozer, Daniel J.
Format: Article
Language:English
Subjects:
Cerebral Cortex - drug effects
Cerebral Cortex - pathology
cortex
cortical lesions
fixation effects
Fixatives - pharmacology
Formaldehyde - pharmacology
Humans
Magnetic Resonance Imaging
magnetization transfer
Middle Aged
multiple sclerosis
Multiple Sclerosis - pathology
postmortem
Postmortem Changes
Tissue Fixation
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Summary:Purpose To investigate changes in magnetic resonance imaging (MRI) indices following formalin fixation of postmortem multiple sclerosis (MS) cortical gray matter (CGM). Postmortem MS brain is being used to establish pathological correlates of changes detected using MRI, with recent emphasis on CGM. Fixation induces tissue alterations that may confound inference of in vivo observations from MRI/histology correlation studies. Materials and Methods T2‐weighted scans were obtained alongside quantitative T1, magnetization transfer ratio (MTR), and macromolecular proton fraction (fB) measurements before and after formalin fixation of 15 postmortem brain samples. Type and size of CGM lesions (CGML) was identified on sections immunostained for myelin basic protein. Results MRI indices obtained in unfixed MS CGM were similar to values obtained in subjects with MS in vivo. Fixation led to reduction in T1 (617 msec [standard deviation = 114] vs. 1156 msec [216]) and MTR (24.1 [3.3] percent units [pu] vs. 29.1 [2.5] pu) and increase in fB (5.4 [0.7] pu vs. 3.2 [2.3] pu) (all P < 0.01). The proportion of CGM affected by demyelination did not alter the MRI data. Conclusion MRI indices in the CGM are significantly altered following tissue fixation. J. Magn. Reson. Imaging 2010;32:1054–1060. © 2010 Wiley‐Liss, Inc.
ISSN:1053-1807
1522-2586
DOI:10.1002/jmri.22381