Successful adoptive transfer and in vivo expansion of haploidentical γδ T cells

The primary aim of this pilot study was to determine the feasibility and safety of an adoptive transfer and in vivo expansion of human haploidentical γδ T lymphocytes. Patients with advanced haematological malignancies who are not eligible for allogeneic transplantation received peripheral blood mon...

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Bibliographic Details
Published in:Journal of translational medicine 2014-02, Vol.12 (1), p.45-45
Main Authors: Wilhelm, Martin, Smetak, Manfred, Schaefer-Eckart, Kerstin, Kimmel, Brigitte, Birkmann, Josef, Einsele, Hermann, Kunzmann, Volker
Format: Article
Language:English
Subjects:
Adoptive Transfer - adverse effects
Adult
Aged
Cell Proliferation
Humans
Male
Receptors, Antigen, T-Cell, gamma-delta - immunology
T-Lymphocytes - cytology
Treatment Outcome
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Summary:The primary aim of this pilot study was to determine the feasibility and safety of an adoptive transfer and in vivo expansion of human haploidentical γδ T lymphocytes. Patients with advanced haematological malignancies who are not eligible for allogeneic transplantation received peripheral blood mononuclear cells from half-matched family donors. For that, a single unstimulated leukapheresis product was incubated with both the anti-CD4 and anti-CD8 antibodies conjugated to paramagnetic particles. The depletion procedure was performed on a fully automated CliniMACS device according to the manufacturer's instructions. On average, patients received 2.17 × 10⁶/kg (range 0.9-3.48) γδ T cells with
ISSN:1479-5876
1479-5876
DOI:10.1186/1479-5876-12-45