Alterations in composition and diversity of the intestinal microbiota in patients with diarrhea-predominant irritable bowel syndrome

Background  The intestinal microbiota has been implicated in the pathophysiology of irritable bowel syndrome (IBS). Due to the variable resolutions of techniques used to characterize the intestinal microbiota, and the heterogeneity of IBS, the defined alterations of the IBS intestinal microbiota are...

Full description

Saved in:
Bibliographic Details
Published in:Neurogastroenterology and motility 2012-06, Vol.24 (6), p.521-e248
Main Authors: Carroll, I. M., Ringel-Kulka, T., Siddle, J. P., Ringel, Y.
Format: Article
Language:English
Subjects:
16S rRNA gene
Adult
Aged
Diarrhea - genetics
Diarrhea - microbiology
DNA, Bacterial - analysis
DNA, Bacterial - genetics
Faecalibacteium prausnitzii
Feces - chemistry
Feces - microbiology
Female
Humans
intestinal microbiota
Intestines - chemistry
Intestines - microbiology
irritable bowel syndrome
Irritable Bowel Syndrome - genetics
Irritable Bowel Syndrome - microbiology
Male
Metagenome - genetics
Middle Aged
pyrosequencing
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background  The intestinal microbiota has been implicated in the pathophysiology of irritable bowel syndrome (IBS). Due to the variable resolutions of techniques used to characterize the intestinal microbiota, and the heterogeneity of IBS, the defined alterations of the IBS intestinal microbiota are inconsistent. We analyzed the composition of the intestinal microbiota in a defined subgroup of IBS patients (diarrhea‐predominant IBS, D‐IBS) using a technique that provides the deepest characterization available for complex microbial communities. Methods  Fecal DNA was isolated from 23 D‐IBS patients and 23 healthy controls (HC). Variable regions V1–V3 and V6 of the 16S rRNA gene were amplified from all samples. PCR products were sequenced using 454 high throughput sequencing. The composition, diversity and richness of microbial communities were determined and compared between D‐IBS and HC using the quantitative insights into microbial ecology pipeline. Key Results  The contribution of bacterial groups to the composition of the intestinal microbiota differed between D‐IBS and HC. D‐IBS patients had significantly higher levels of Enterobacteriaceae (P = 0.03), and lower levels of Fecalibacterium genera (P = 0.04) compared to HC. β‐Diversity values demonstrated significantly lower levels of UniFrac distances in HC compared to D‐IBS patients. The richness of 16S rRNA sequences was significantly decreased in D‐IBS patients (P 
ISSN:1350-1925
1365-2982
DOI:10.1111/j.1365-2982.2012.01891.x