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The Arabidopsis CALLOSE DEFECTIVE MICROSPORE1 Gene Is Required for Male Fertility through Regulating Callose Metabolism during Microsporogenesis1[W][OPEN]
Accurate regulation of callose metabolism during microsporogenesis is critical for plant male fertility . During angiosperm microsporogenesis, callose serves as a temporary wall to separate microsporocytes and newly formed microspores in the tetrad. Abnormal callose deposition and dissolution can le...
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Published in: | Plant physiology (Bethesda) 2014-04, Vol.164 (4), p.1893-1904 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Online Access: | Get full text |
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Summary: | Accurate regulation of callose metabolism during microsporogenesis is
critical for plant male fertility
.
During angiosperm microsporogenesis, callose serves as a temporary wall to separate
microsporocytes and newly formed microspores in the tetrad. Abnormal callose
deposition and dissolution can lead to degeneration of developing microspores.
However, genes and their regulation in callose metabolism during microsporogenesis
still remain largely unclear. Here, we demonstrated that the Arabidopsis
(
Arabidopsis thaliana
)
CALLOSE DEFECTIVE
MICROSPORE1
(
CDM1
) gene, encoding a tandem CCCH-type
zinc finger protein, plays an important role in regulation of callose metabolism in
male meiocytes and in integrity of newly formed microspores. First, quantitative
reverse transcription PCR and in situ hybridization analyses showed that the
CDM1
gene was highly expressed in meiocytes and the tapetum from
anther stages 4 to 7. In addition, a transfer DNA insertional
cdm1
mutant was completely male sterile. Moreover, light microscopy of anther sections
revealed that microspores in the mutant anther were initiated, and then degenerated
soon afterward with callose deposition defects, eventually leading to male sterility.
Furthermore, transmission electron microscopy demonstrated that pollen exine
formation was severely affected in the
cdm1
mutant. Finally, we
found that the
cdm1
mutation affected the expression of callose
synthesis genes (
CALLOSE SYNTHASE5
and
CALLOSE
SYNTHASE12
) and potential callase-related genes (
A6
and
MYB80
), as well as three other putative β-1,3-glucanase
genes. Therefore, we propose that the
CDM1
gene regulates callose
metabolism during microsporogenesis, thereby promoting Arabidopsis male
fertility. |
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ISSN: | 0032-0889 1532-2548 |
DOI: | 10.1104/pp.113.233387 |