The Impact of Chronic Intrauterine Inflammation on the Physiologic and Neurodevelopmental Consequences of Intermittent Umbilical Cord Occlusion in Fetal Sheep

Objective: To determine the effect of intrauterine inflammation on fetal responses to umbilical cord occlusion (UCO). Study Design: In pregnant sheep, lipopolysaccharide (LPS) or saline (SAL) was infused intra-amniotically for 4 weeks from 80 days of gestation (d). At 110 d, fetuses were instrumente...

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Published in:Reproductive sciences (Thousand Oaks, Calif.) Calif.), 2014-05, Vol.21 (5), p.658-670
Main Authors: Nitsos, Ilias, Newnham, John P., Rees, Sandra M., Harding, Richard, Moss, Timothy J. M.
Format: Article
Language:English
Subjects:
Animals
Brain - embryology
Brain - metabolism
Brain - pathology
Chronic Disease
Embryology
Female
Fetal Development - physiology
Fetal Hypoxia - blood
Fetal Hypoxia - pathology
Fetus
Inflammation - blood
Inflammation - pathology
Medicine & Public Health
Obstetrics/Perinatology/Midwifery
Original
Original Article
Pregnancy
Reproductive Medicine
Sheep
Umbilical Cord - pathology
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Summary:Objective: To determine the effect of intrauterine inflammation on fetal responses to umbilical cord occlusion (UCO). Study Design: In pregnant sheep, lipopolysaccharide (LPS) or saline (SAL) was infused intra-amniotically for 4 weeks from 80 days of gestation (d). At 110 d, fetuses were instrumented for UCOs (5 × 2-minutes, 30-minute intervals: LPS + UCO, n = 6; SAL + UCO, n = 8) or no UCO (sham, n = 6) on 117 and 118 d. Tissues were collected at 126 d. Results: Fetal physiological responses to UCO were similar between LPS + UCO and SAL + UCO. Histologic chorioamnionitis and increased amniotic fluid interleukin 8 (IL-8) were observed in LPS + UCO pregnancies (versus SAL + UCO, P < .05). CNPase-positive oligodendrocyte number in the cerebral white matter was lower in LPS + UCO and SAL + UCO than sham (P < .05); there was no effect on astrocytes or activated microglia/macrophages. Two of the SAL + UCO fetuses had white matter lesions; none were observed in LPS + UCO or sham. Conclusion: Chronic pre-existing intrauterine inflammation did not exacerbate fetal brain injury induced by intermittent UCO.
ISSN:1933-7191
1933-7205
DOI:10.1177/1933719111399928