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Microarray Analysis of Serum mRNA in Patients with Head and Neck Squamous Cell Carcinoma at Whole-Genome Scale
With the increasing demand for noninvasive approaches in monitoring head and neck cancer, circulating nucleic acids have been shown to be a promising tool. We focused on the global transcriptome of serum samples of head and neck squamous cell carcinoma (HNSCC) patients in comparison with healthy ind...
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| Published in: | BioMed research international 2014-01, Vol.2014 (2014), p.1-10 |
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| creator | Šáchová, Jana Čapková, Markéta Strnad, Hynek Kolář, Michal Hroudová, Miluše Chovanec, Martin Čada, Zdeněk Šteffl, Martin Valach, Jaroslav Kastner, Jan Vlček, Čestmír Smetana, Karel Plzak, Jan |
| description | With the increasing demand for noninvasive approaches in monitoring head and neck cancer, circulating nucleic acids have been shown to be a promising tool. We focused on the global transcriptome of serum samples of head and neck squamous cell carcinoma (HNSCC) patients in comparison with healthy individuals. We compared gene expression patterns of 36 samples. Twenty-four participants including 16 HNSCC patients (from 12 patients we obtained blood samples 1 year posttreatment) and 8 control subjects were recruited. The Illumina HumanWG-6 v3 Expression BeadChip was used to profile and identify the differences in serum mRNA transcriptomes. We found 159 genes to be significantly changed (Storey’s P value |
| doi_str_mv | 10.1155/2014/408683 |
| format | article |
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We focused on the global transcriptome of serum samples of head and neck squamous cell carcinoma (HNSCC) patients in comparison with healthy individuals. We compared gene expression patterns of 36 samples. Twenty-four participants including 16 HNSCC patients (from 12 patients we obtained blood samples 1 year posttreatment) and 8 control subjects were recruited. The Illumina HumanWG-6 v3 Expression BeadChip was used to profile and identify the differences in serum mRNA transcriptomes. We found 159 genes to be significantly changed (Storey’s P value <0.05) between normal and cancer serum specimens regardless of factors including p53 and B-cell lymphoma family members (Bcl-2, Bcl-XL). In contrast, there was no difference in gene expression between samples obtained before and after surgery in cancer patients. We suggest that microarray analysis of serum cRNA in patients with HNSCC should be suitable for refinement of early stage diagnosis of disease that can be important for development of new personalized strategies in diagnosis and treatment of tumours but is not suitable for monitoring further development of disease.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2014/408683</identifier><identifier>PMID: 24864240</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Puplishing Corporation</publisher><subject>Adult ; Aged ; Apoptosis ; Apoptosis - genetics ; Bioinformatics ; Biomarkers ; Biopsy ; Cancer ; Carcinoma, Squamous Cell - blood ; Carcinoma, Squamous Cell - genetics ; Carcinoma, Squamous Cell - pathology ; Case-Control Studies ; Demography ; DNA microarrays ; Epigenetics ; Female ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Genome, Human - genetics ; Head and neck cancer ; Head and Neck Neoplasms - blood ; Head and Neck Neoplasms - genetics ; Head and Neck Neoplasms - pathology ; Humans ; Male ; Medical prognosis ; Medicine ; Messenger RNA ; Microarray Analysis ; Middle Aged ; Otolaryngology ; Patients ; Principal Component Analysis ; Principal components analysis ; RNA, Messenger - blood ; RNA, Messenger - genetics ; Signal Transduction - genetics ; Squamous Cell Carcinoma of Head and Neck ; Studies ; Surgery ; Tumor Suppressor Protein p53 - metabolism</subject><ispartof>BioMed research international, 2014-01, Vol.2014 (2014), p.1-10</ispartof><rights>Copyright © 2014 Markéta Čapková et al.</rights><rights>COPYRIGHT 2014 John Wiley & Sons, Inc.</rights><rights>Copyright © 2014 Markéta Capková et al. Markéta Capková et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2014 Markéta Čapková et al. 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c527t-fb49c2d74e99f2aad3923055318cd64c945310a4e6aa2df91738647a9686f6fe3</citedby><cites>FETCH-LOGICAL-c527t-fb49c2d74e99f2aad3923055318cd64c945310a4e6aa2df91738647a9686f6fe3</cites><orcidid>0000-0001-5335-5444 ; 0000-0003-3710-4455 ; 0000-0002-7200-9755 ; 0000-0003-3389-658X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1546414590/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1546414590?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,25751,27922,27923,37010,37011,44588,74896</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24864240$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Betka, Jan</contributor><creatorcontrib>Šáchová, Jana</creatorcontrib><creatorcontrib>Čapková, Markéta</creatorcontrib><creatorcontrib>Strnad, Hynek</creatorcontrib><creatorcontrib>Kolář, Michal</creatorcontrib><creatorcontrib>Hroudová, Miluše</creatorcontrib><creatorcontrib>Chovanec, Martin</creatorcontrib><creatorcontrib>Čada, Zdeněk</creatorcontrib><creatorcontrib>Šteffl, Martin</creatorcontrib><creatorcontrib>Valach, Jaroslav</creatorcontrib><creatorcontrib>Kastner, Jan</creatorcontrib><creatorcontrib>Vlček, Čestmír</creatorcontrib><creatorcontrib>Smetana, Karel</creatorcontrib><creatorcontrib>Plzak, Jan</creatorcontrib><title>Microarray Analysis of Serum mRNA in Patients with Head and Neck Squamous Cell Carcinoma at Whole-Genome Scale</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>With the increasing demand for noninvasive approaches in monitoring head and neck cancer, circulating nucleic acids have been shown to be a promising tool. 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We suggest that microarray analysis of serum cRNA in patients with HNSCC should be suitable for refinement of early stage diagnosis of disease that can be important for development of new personalized strategies in diagnosis and treatment of tumours but is not suitable for monitoring further development of disease.</description><subject>Adult</subject><subject>Aged</subject><subject>Apoptosis</subject><subject>Apoptosis - genetics</subject><subject>Bioinformatics</subject><subject>Biomarkers</subject><subject>Biopsy</subject><subject>Cancer</subject><subject>Carcinoma, Squamous Cell - blood</subject><subject>Carcinoma, Squamous Cell - genetics</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Case-Control Studies</subject><subject>Demography</subject><subject>DNA microarrays</subject><subject>Epigenetics</subject><subject>Female</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Genome, Human - genetics</subject><subject>Head and neck cancer</subject><subject>Head and Neck Neoplasms - 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We focused on the global transcriptome of serum samples of head and neck squamous cell carcinoma (HNSCC) patients in comparison with healthy individuals. We compared gene expression patterns of 36 samples. Twenty-four participants including 16 HNSCC patients (from 12 patients we obtained blood samples 1 year posttreatment) and 8 control subjects were recruited. The Illumina HumanWG-6 v3 Expression BeadChip was used to profile and identify the differences in serum mRNA transcriptomes. We found 159 genes to be significantly changed (Storey’s P value <0.05) between normal and cancer serum specimens regardless of factors including p53 and B-cell lymphoma family members (Bcl-2, Bcl-XL). In contrast, there was no difference in gene expression between samples obtained before and after surgery in cancer patients. 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| fulltext | fulltext |
| identifier | ISSN: 2314-6133 |
| ispartof | BioMed research international, 2014-01, Vol.2014 (2014), p.1-10 |
| issn | 2314-6133 2314-6141 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4017838 |
| source | Publicly Available Content Database (Proquest) (PQ_SDU_P3); Wiley Open Access |
| subjects | Adult Aged Apoptosis Apoptosis - genetics Bioinformatics Biomarkers Biopsy Cancer Carcinoma, Squamous Cell - blood Carcinoma, Squamous Cell - genetics Carcinoma, Squamous Cell - pathology Case-Control Studies Demography DNA microarrays Epigenetics Female Gene Expression Profiling Gene Expression Regulation, Neoplastic Genome, Human - genetics Head and neck cancer Head and Neck Neoplasms - blood Head and Neck Neoplasms - genetics Head and Neck Neoplasms - pathology Humans Male Medical prognosis Medicine Messenger RNA Microarray Analysis Middle Aged Otolaryngology Patients Principal Component Analysis Principal components analysis RNA, Messenger - blood RNA, Messenger - genetics Signal Transduction - genetics Squamous Cell Carcinoma of Head and Neck Studies Surgery Tumor Suppressor Protein p53 - metabolism |
| title | Microarray Analysis of Serum mRNA in Patients with Head and Neck Squamous Cell Carcinoma at Whole-Genome Scale |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T13%3A49%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Microarray%20Analysis%20of%20Serum%20mRNA%20in%20Patients%20with%20Head%20and%20Neck%20Squamous%20Cell%20Carcinoma%20at%20Whole-Genome%20Scale&rft.jtitle=BioMed%20research%20international&rft.au=%C5%A0%C3%A1chov%C3%A1,%20Jana&rft.date=2014-01-01&rft.volume=2014&rft.issue=2014&rft.spage=1&rft.epage=10&rft.pages=1-10&rft.issn=2314-6133&rft.eissn=2314-6141&rft_id=info:doi/10.1155/2014/408683&rft_dat=%3Cgale_pubme%3EA427022939%3C/gale_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c527t-fb49c2d74e99f2aad3923055318cd64c945310a4e6aa2df91738647a9686f6fe3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1546414590&rft_id=info:pmid/24864240&rft_galeid=A427022939&rfr_iscdi=true |