A Role for WDR5 in Integrating Threonine 11 Phosphorylation to Lysine 4 Methylation on Histone H3 during Androgen Signaling and in Prostate Cancer

• Published in: Molecular cell 2014-05, Vol.54 (4), p.613-625
• Main Authors: Kim, Ji-Young, Banerjee, Taraswi, Vinckevicius, Aurimas, Luo, Qianyi, Parker, J. Brandon, Baker, Mairead R., Radhakrishnan, Ishwar, Wei, Jian-Jun, Barish, Grant D., Chakravarti, Debabrata
• Format: Article
• Language: English
• Subjects: Androgens - metabolism; Cell Line, Tumor; Cell Proliferation; Chromatin - metabolism; Epigenesis, Genetic; Gene Expression Regulation, Neoplastic; Gene Knockdown Techniques; HeLa Cells; Histone-Lysine N-Methyltransferase - genetics; Histone-Lysine N-Methyltransferase - metabolism; Histones - genetics; Histones - metabolism; Humans; Lysine - metabolism; Male; Methylation; Myeloid-Lymphoid Leukemia Protein - genetics; Myeloid-Lymphoid Leukemia Protein - metabolism; Phosphorylation; Prostatic Neoplasms - genetics; Prostatic Neoplasms - metabolism; Prostatic Neoplasms - pathology; Protein Kinase C - genetics; Protein Kinase C - metabolism; Receptors, Androgen - genetics; Receptors, Androgen - metabolism; Signal Transduction; Threonine - genetics; Threonine - metabolism
• ISSN: 1097-2765; 1097-4164
• DOI: 10.1016/j.molcel.2014.03.043

Upon androgen stimulation, PKN1-mediated histone H3 threonine 11 phosphorylation (H3T11P) promotes AR target gene activation. However, the underlying mechanism is not completely understood. Here, we show that WDR5, a subunit of the SET1/MLL complex, interacts with H3T11P, and this interaction facilitates the recruitment of the MLL1 complex and subsequent H3K4 tri-methylation (H3K4me3). Using ChIP-seq, we find that androgen stimulation results in a 6-fold increase in the number of H3T11P-marked regions and induces WDR5 colocalization to one third of H3T11P-enriched promoters, thus establishing a genome-wide relationship between H3T11P and recruitment of WDR5. Accordingly, PKN1 knockdown or chemical inhibition severely blocks WDR5 chromatin association and H3K4me3 on AR target genes. Finally, WDR5 is critical in prostate cancer cell proliferation and is hyperexpressed in human prostate cancers. Together, these results identify WDR5 as a critical epigenomic integrator of histone phosphorylation and methylation and as a major driver of androgen-dependent prostate cancer cell proliferation. [Display omitted] •H3T11P facilitates WDR5 and MLL1 complex recruitment•Genome wide localization of WDR5 and H3T11P analyzed•A mechanism involving WDR5 and H3T11P in AR target gene activation is established•WDR5 is overexpressed in prostate cancer and critical for cancer cell proliferation H3T11 phosphorylation is important for androgen-dependent gene expression in prostate cancer, but the basis for this is not clear. Kim et al. report that upon androgen signaling, WDR5 binds H3T11P to recruit SET1/MLL for H3K4 methylation, indicating that WDR5 mediates crosstalk between histone marks involved in cancer proliferation.