A cancer-related protein 14-3-3ζ is a potential tumor-associated antigen in immunodiagnosis of hepatocellular carcinoma

Hepatocellular carcinoma (HCC) is the fifth most common cancer and the third leading cause of cancer-related deaths worldwide. Serum alpha-fetoprotein (AFP) is the conventional biomarker currently used in clinical diagnosis of this malignancy. However, AFP is not reliable for early diagnosis, and es...

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Bibliographic Details
Published in:Tumor biology 2014-05, Vol.35 (5), p.4247-4256
Main Authors: Liu, Mei, Liu, Xinxin, Ren, Pengfei, Li, Jitian, Chai, Yurong, Zheng, Su-Jun, Chen, Yu, Duan, Zhong-Ping, Li, Ning, Zhang, Jian-Ying
Format: Article
Language:English
Subjects:
14-3-3 Proteins - immunology
Adult
Aged
Antigens, Neoplasm - immunology
Autoantibodies - blood
Biomarkers, Tumor - blood
Biomedical and Life Sciences
Biomedicine
Cancer Research
Carcinoma, Hepatocellular - diagnosis
Carcinoma, Hepatocellular - immunology
Cell Line, Tumor
Female
Fluorescent Antibody Technique, Indirect
Humans
Liver Neoplasms - diagnosis
Liver Neoplasms - immunology
Male
Middle Aged
Research Article
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Summary:Hepatocellular carcinoma (HCC) is the fifth most common cancer and the third leading cause of cancer-related deaths worldwide. Serum alpha-fetoprotein (AFP) is the conventional biomarker currently used in clinical diagnosis of this malignancy. However, AFP is not reliable for early diagnosis, and especially the sensitivity and specificity of AFP in HCC diagnosis are not optimal. Early detection of HCC is an important issue because of the very poor prognosis and usually no more than 6 months survival after diagnosis. Therefore, there is a need for the development of more sensitive and specific methods that can supplement AFP in the early detection of this cancer. In this study, autoantibody responses to 14-3-3ζ in HCC were evaluated by enzyme-linked immunosorbent assay (ELISA), western blot, and indirect immunofluorescence assay. Immunohistochemistry (IHC) with tissue array slides was also performed to analyze protein expression of 14-3-3ζ in HCC and control tissues. The prevalence of autoantibodies against 14-3-3ζ was 16.7 % (28/168) in HCC, which was significantly higher than that in liver cirrhosis (LC), chronic hepatitis (CH), and normal human sera (NHS) ( P  
ISSN:1010-4283
1423-0380
DOI:10.1007/s13277-013-1555-8