Aberrant DNA methylation patterns in diabetic nephropathy

Background The aim of this study was to evaluate whether global levels of DNA methylation status were associated with albuminuria and progression of diabetic nephropathy in a case-control study of 123 patients with type 2 diabetes- 53 patients with albuminuria and 70 patients without albuminuria. Me...

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Published in:Journal of diabetes and metabolic disorders 2014-06, Vol.13 (1), p.69-69, Article 69
Main Authors: Maghbooli, Zhila, Larijani, Bagher, Emamgholipour, Solaleh, Amini, Manochehr, Keshtkar, Abbasali, Pasalar, Parvin
Format: Article
Language:English
Subjects:
Antihypertensive drugs
Diabetes
Endocrinology
Epigenetic inheritance
Genetic aspects
Genetic research
High performance liquid chromatography
Medical research
Medicine
Medicine & Public Health
Medicine, Experimental
Metabolic Diseases
Pyrimidines
Research Article
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Summary:Background The aim of this study was to evaluate whether global levels of DNA methylation status were associated with albuminuria and progression of diabetic nephropathy in a case-control study of 123 patients with type 2 diabetes- 53 patients with albuminuria and 70 patients without albuminuria. Methods The 5-methyl cytosine content was assessed by reverse phase high pressure liquid chromatography (RP-HPLC) of peripheral blood mononuclear cells to determine individual global DNA methylation status in two groups. Results Global DNA methylation levels were significantly higher in patients with albuminuria compared with those in normal range of albuminuria (p = 0.01). There were significant differences in global levels of DNA methylation in relation to albuminuria (p = 0.028) and an interesting pattern of increasing global levels of DNA methylation in terms of albuminuria severity. In patients with micro- and macro albuminuria, we found no significant correlations between global DNA methylation levels and duration of diabetes (p > 0.05). In both sub groups, there were not significant differences between global DNA methylation levels with good and poor glycaemic control (p > 0.05). In addition, in patients with albuminuria, no differences in DNA methylation levels were observed between patients with and without other risk factors including age, gender, hypertension, dyslipidaemia and obesity. Conclusions These data may be helpful in further studies to develop novel biomarkers and new strategies for clinical care of patients at risk of diabetic nephropathy.
ISSN:2251-6581
2251-6581
DOI:10.1186/2251-6581-13-69