Bromophenacyl bromide, a phospholipase A2 inhibitor attenuates chemically induced gastroduodenal ulcers in rats

• Published in: World journal of gastroenterology : WJG 2006-09, Vol.12 (36), p.5798-5804
• Main Authors: Tariq, Mohammad, Elfaki, Ibrahim, Khan, Haseeb-Ahmad, Arshaduddin, Mohammad, Sobki, Samia, Al Moutaery, Meshal
• Format: Article
• Language: English
• Subjects: Acetophenones - pharmacology; Animals; Anti-Ulcer Agents - pharmacology; Basic Research; Cysteamine; Ethanol; Female; Gastric Acid - metabolism; Gastric Mucosa - drug effects; Gastric Mucosa - metabolism; Gastric Mucosa - pathology; Intestinal Mucosa - drug effects; Intestinal Mucosa - metabolism; Intestinal Mucosa - pathology; Male; Peptic Ulcer - chemically induced; Peptic Ulcer - metabolism; Peptic Ulcer - pathology; Peptic Ulcer - prevention & control; Peroxidase - metabolism; Phospholipases A - antagonists & inhibitors; Phospholipases A2; Rats; Rats, Wistar; Sulfhydryl Compounds - metabolism; 动物实验; 溴化物; 磷脂酶A2; 胃十二指肠溃疡
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v12.i36.5798

AIM： To study the effect of bromophenacyl bromide （BPB）, a phospholipase A2 inhibitor on gastric secretion and to protect chemically induced gastric and duodenal ulcers in rats.METHODS： Acid secretion studies were undertaken in pylorus-ligated rats with BPB treatment （0, 5, 15 and 45 mg/kg）. Gastric and duodenal lesions in the rats were induced by ethanol and cysteamine respectively. The levels of gastric wall mucus, nonprotein sulfhydryls （NP-SH） and myeloperoxidase （MPO） were also measured in the glandular stomach of rats following ethanol induced gastric lesions.RESULTS： BPB produced a dose-dependent inhibition of gastric acid secretion and acidity in rats. Pretreatment with BPB significantly attenuated the formation of ethanol induced gastric lesion. BPB also protected intestinal mucosa against cysteamine-induced duodenal ulcers. The antiulcer activity of BPB was associated with significant inhibition of ethanol-induced depletion of gastric wall mucus, NP-SH and MPO. These findings pointed towards the mediation of sulfhydryls in BPB induced gastrointestinal cytoprotection.CONCLUSION： BPB possesses significant antiulcer and cytoprotective activity against experimentally induced gastroduodenal lesions.