Genomic Organization and Identification of Promoter Regions for the BDNF Gene in the Pond Turtle Trachemys scripta elegans

Brain-derived neurotrophic factor (BDNF) is an important regulator of neuronal development and synaptic function. The BDNF gene undergoes significant activity-dependent regulation during learning. Here, we identified the BDNF promoter regions, transcription start sites, and potential regulatory sequ...

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Bibliographic Details
Published in:Journal of molecular neuroscience 2014-08, Vol.53 (4), p.626-636
Main Authors: Ambigapathy, Ganesh, Zheng, Zhaoqing, Keifer, Joyce
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Binding sites
Biomedical and Life Sciences
Biomedicine
Brain-derived neurotrophic factor
Brain-Derived Neurotrophic Factor - genetics
Brain-Derived Neurotrophic Factor - metabolism
Cell Biology
Cell Line, Tumor
Classical conditioning
Cloning
Cyclic AMP Response Element-Binding Protein - metabolism
Exons
Fibroblasts
Gene expression
Genome
Genomes
Humans
Laboratory animals
Mice
Molecular Sequence Data
Neuroblastoma
Neurochemistry
Neurology
Neurosciences
NIH 3T3 Cells
Promoter Regions, Genetic
Protein expression
Proteins
Proteomics
Reptilian Proteins - genetics
Reptilian Proteins - metabolism
Trachemys scripta elegans
Transcription factors
Turtles - genetics
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Summary:Brain-derived neurotrophic factor (BDNF) is an important regulator of neuronal development and synaptic function. The BDNF gene undergoes significant activity-dependent regulation during learning. Here, we identified the BDNF promoter regions, transcription start sites, and potential regulatory sequences for BDNF exons I–III that may contribute to activity-dependent gene and protein expression in the pond turtle Trachemys scripta elegans ( tBDNF ). By using transfection of BDNF promoter/luciferase plasmid constructs into human neuroblastoma SHSY5Y cells and mouse embryonic fibroblast NIH3T3 cells, we identified the basal regulatory activity of promoter sequences located upstream of each tBDNF exon, designated as pBDNFI–III. Further, through chromatin immunoprecipitation (ChIP) assays, we detected CREB binding directly to exon I and exon III promoters, while BHLHB2, but not CREB, binds within the exon II promoter. Elucidation of the promoter regions and regulatory protein binding sites in the tBDNF gene is essential for understanding the regulatory mechanisms that control tBDNF gene expression.
ISSN:0895-8696
1559-1166
DOI:10.1007/s12031-014-0229-3