The Receptor-Like Kinase SIT1 Mediates Salt Sensitivity by Activating MAPK3/6 and Regulating Ethylene Homeostasis in Rice

High salinity causes growth inhibition and shoot bleaching in plants that do not tolerate high salt (glycophytes), including most crops. The molecules affected directly by salt and linking the extracellular stimulus to intracellular responses remain largely unknown. Here, we demonstrate that rice (O...

Full description

Saved in:
Bibliographic Details
Published in:The Plant cell 2014-06, Vol.26 (6), p.2538-2553
Main Authors: Li, Chen-Hui, Wang, Geng, Zhao, Ji-Long, Zhang, Li-Qing, Ai, Lian-Feng, Han, Yong-Feng, Sun, Da-Ye, Zhang, Sheng-Wei, Sun, Ying
Format: Article
Language:English
Subjects:
Epidermal cells
Phosphorylation
Plant cells
Plant roots
Plants
Rice
Salt tolerance
Salts
Seedlings
Survival rates
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:High salinity causes growth inhibition and shoot bleaching in plants that do not tolerate high salt (glycophytes), including most crops. The molecules affected directly by salt and linking the extracellular stimulus to intracellular responses remain largely unknown. Here, we demonstrate that rice (Oryza sativa) Salt Intolerance 1 [SITI), a lectin receptor-like kinase expressed mainly in root epidermal cells, mediates salt sensitivity. NaCI rapidly activates SIT1, and in the presence of salt, as SIT1 kinase activity increased, plant survival decreased. Rice MPK3 and MPK6 function as the downstream effectors of SITI. SIT1 phosphorylates MPK3 and 6, and their activation by salt requires SITI. SIT1 mediates ethylene production and salt-induced ethylene signaling. SIT1 promotes accumulation of reactive oxygen species (ROS), leading to growth inhibition and plant death under salt stress, which occurred in an MPK3/6-and ethylene signaling-dependent manner in Arabidopsis thaliana. Our findings demonstrate the existence of a SIT1-MPK3/6 cascade that mediates salt sensitivity by affecting ROS and ethylene homeostasis and signaling. These results provide important information for engineering salt-tolerant crops.
ISSN:1040-4651
1532-298X
DOI:10.1105/tpc.114.125187