Neutrophil depletion-but not prevention of Kupffer cell activation-decreases the severity of cerulein-induced acute pancreatitis

• Published in: World journal of gastroenterology : WJG 2006-02, Vol.12 (8), p.1219-1224
• Main Authors: Pastor, Catherine M, Vonlaufen, Alain, Georgi, Fabianna, Hadengue, Antoine, Morel, Philippe, Frossard, Jean-Louis
• Format: Article
• Language: English
• Subjects: Acute Disease; Amylases - blood; Animals; Basic Research; Cell Count; Ceruletide; Gadolinium - pharmacology; Interleukins - analysis; Interleukins - blood; Kupffer Cells - drug effects; Kupffer Cells - physiology; Liver - chemistry; Liver - pathology; Lung - chemistry; Lung - pathology; Lung - physiopathology; Macrophage Activation; Male; Mice; Mice, Inbred C57BL; Neutrophil Activation; Neutrophils - immunology; Pancreas - chemistry; Pancreas - pathology; Pancreatitis - chemically induced; Pancreatitis - immunology; Pancreatitis - pathology; Peroxidase - analysis; Random Allocation; Severity of Illness Index; 中性白细胞; 疾病预防; 胰腺炎; 蛙皮缩胆囊肽
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v12.i8.1219

AIM： To determine whether neutrophil depletion and Kupffer cell inhibition might combine their protective effects to decrease the severity of acute pancreatitis. METHODS： Nice had cerulein administration to induce acute pancreatitis and were pretreated with either anti-mouse neutrophil serum or gadolinium chloride （GdCh） to prevent Kupffer cell activation, or both treatments. Injury was assessed in pancreas and lungs. Myeloperoxidases （MPO） assessed neutrophil infiltration. Interleukin-6 （IL-6） and IL-10 were measured in serum, pancreas, lungs and liver. RESULTS： In mice with acute pancreatitis, neutrophil depletion reduced the severity of pancreatitis and pancreatitis-associated lung injury. Kupffer cell inactivation by GdCh had less protective effect, although IL-6 and IL-10 concentrations were significantly decreased. The protective treatment brought by neutrophil depletion was not enhanced by Kupffer cell inactivation and both treatments did not combine their protective effects. CONCLUSION： Our results confirm the role of activated neutrophils in aggravating organ injury in acute pancreatitis while the role of Kupffer cell activation is less obvious.