Bordetella pertussis Lipid A Glucosamine Modification Confers Resistance to Cationic Antimicrobial Peptides and Increases Resistance to Outer Membrane Perturbation

Bordetella pertussis, the causative agent of whooping cough, has many strategies for evading the human immune system. Lipopolysaccharide (LPS) is an important Gram-negative bacterial surface structure that activates the immune system via Toll-like receptor 4 and enables susceptibility to cationic an...

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Bibliographic Details
Published in:Antimicrobial agents and chemotherapy 2014-08, Vol.58 (8), p.4931-4934
Main Authors: SHAH, Nita R, HANCOCK, Robert E. W, FERNANDEZ, Rachel C
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Anti-Bacterial Agents - pharmacology
Antibacterial agents
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antimicrobial Cationic Peptides
Antimicrobial Cationic Peptides - pharmacology
Biological and medical sciences
Bordetella pertussis
Bordetella pertussis - chemistry
Bordetella pertussis - drug effects
Bordetella pertussis - growth & development
Bordetella pertussis - metabolism
Cell Membrane
Cell Membrane - chemistry
Cell Membrane - drug effects
Cell Membrane - metabolism
Drug Resistance, Bacterial
Glucosamine
Glucosamine - chemistry
Glucosamine - metabolism
Lipid A
Lipid A - chemistry
Lipid A - metabolism
Medical sciences
Microbial Sensitivity Tests
Molecular Sequence Data
Pharmacology. Drug treatments
Polymyxins - pharmacology
Susceptibility
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Summary:Bordetella pertussis, the causative agent of whooping cough, has many strategies for evading the human immune system. Lipopolysaccharide (LPS) is an important Gram-negative bacterial surface structure that activates the immune system via Toll-like receptor 4 and enables susceptibility to cationic antimicrobial peptides (CAMPs). We show modification of the lipid A region of LPS with glucosamine increased resistance to numerous CAMPs, including LL-37. Furthermore, we demonstrate that this glucosamine modification increased resistance to outer membrane perturbation.
ISSN:0066-4804
1098-6596
DOI:10.1128/aac.02590-14