Comparison of biological characteristics of marrow mesenchymal stem cells in hepatitis B patients and normal adults

• Published in: World journal of gastroenterology : WJG 2007-03, Vol.13 (11), p.1743-1746
• Main Authors: Peng, Liang, Li, Hua, Gu, Lin, Peng, Xiao-Mou, Huang, Yang-Su, Gao, Zhi-Liang
• Format: Article
• Language: English
• Subjects: Adult; Antigens, CD34 - metabolism; Bone Marrow Cells - cytology; Bone Marrow Cells - immunology; Bone Marrow Cells - pathology; Case-Control Studies; Cell Culture Techniques - methods; Cell Proliferation; Cells, Cultured; Cellular Senescence; Hepatitis B - immunology; Hepatitis B - pathology; Humans; Hyaluronan Receptors - metabolism; Leukocyte Common Antigens - metabolism; Male; Mesenchymal Stem Cells - cytology; Mesenchymal Stem Cells - immunology; Mesenchymal Stem Cells - pathology; Middle Aged; Rapid Communication; 乙型肝炎患者; 正常人; 比较研究; 生物学特性; 骨髓间叶干细胞
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v13.i11.1743

AIM： To establish a culture system of marrow mesenchymal stem cells （MSCs） from hepatitis B patients and normal adults and to compare their biological characteristics. METHODS： MSCs were isolated from bone marrow in 34 male hepatitis B patients and 15 male normal adults and cultivated in vitro. Their biological characteristics including surface markers, shapes and appearances, growth curves, first passage time and passage generations were compared. RESULTS： Cultivation achievement ratio of hepatitis B patients was lower than that of normal adults, no statistical significance （82.35% vs 100%, P 〉0.05）. Compared with MSCs of normal adults, MSCs of hepatitis B patients presented a statistical lower growth curve, longer first passage time （13.0 ± 1.6 d vs 11.4 ± 1.5 d, P 〈 0.05）, fewer passaging generation numbers （10.5 ± 1.4 generations vs 12.3±1.7 generations, P 〈 0.05）, though both shared same appearances, shapes and surface markers. MSCs in hepatitis B patients would expand, spread out and age more easily and there were more refractive particles in the cytoplasm. CONCLUSION： MSCs from hepatitis B patients can be cultured in vitro. Although their appearance, shape and surface marker are similar to those of MSCs from normal adults, there are differences in their biological characteristics.