Adulteration and cultivation region identification of American ginseng using HPLC coupled with multivariate analysis

• Published in: Journal of pharmaceutical and biomedical analysis 2014-10, Vol.99, p.8-15
• Main Authors: Yu, Chunhao, Wang, Chong-Zhi, Zhou, Chun-Jie, Wang, Bin, Han, Lide, Zhang, Chun-Feng, Wu, Xiao-Hui, Yuan, Chun-Su
• Format: Article
• Language: English
• Subjects: Calibration; Canada; China; Chromatography, High Pressure Liquid - methods; Cultivation region identification; Discriminant Analysis; Drug Contamination; Hierarchical cluster analysis; HPLC-linear discriminant analysis; Multivariate Analysis; Panax - chemistry; Panax - growth & development; Panax quinquefolius; Plant Extracts - analysis; Plant Extracts - standards; Plant Roots - chemistry; Plant Roots - growth & development; Plants, Medicinal; Principal Component Analysis; Reference Standards; Saponins - analysis; Species Specificity; United States
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2014.06.031

•It is difficult to distinguish origins of American ginseng, especially cultivation locations.•We developed HPLC coupled with HCA and PCA methods to identify the Panax species.•We established an HPLC-LDA method to identify American ginseng cultivation regions.•This method was successfully applied to identify the sources of commercial samples. American ginseng (Panax quinquefolius) is originally grown in North America. Due to price difference and supply shortage, American ginseng recently has been cultivated in northern China. Further, in the market, some Asian ginsengs are labeled as American ginseng. In this study, forty-three American ginseng samples cultivated in the USA, Canada or China were collected and 14 ginseng saponins were determined using HPLC. HPLC coupled with hierarchical cluster analysis and principal component analysis was developed to identify the species. Subsequently, an HPLC-linear discriminant analysis was established to discriminate cultivation regions of American ginseng. This method was successfully applied to identify the sources of 6 commercial American ginseng samples. Two of them were identified as Asian ginseng, while 4 others were identified as American ginseng, which were cultivated in the USA (3) and China (1). Our newly developed method can be used to identify American ginseng with different cultivation regions.