The Effect of Interspecific Oocytes on Demethylation of Sperm DNA

In contrast to mice, in sheep no genome-wide demethylation of the paternal genome occurs within the first postfertilization cell cycle. This difference could be due either to an absence of a sheep demethylase activity that is present in mouse ooplasm or to an increased protection of methylated cytos...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2004-05, Vol.101 (20), p.7636-7640
Main Authors: Beaujean, Nathalie, Taylor, Jane E., McGarry, Michelle, Gardner, John O., Wilmut, Ian, Loi, Pasqualino, Ptak, Grazyna, Galli, Cesare, Lazzari, Giovanna, Bird, Adrian, Young, Lorraine E., Meehan, Richard R., Jaenisch, Rudolf
Format: Article
Language:English
Subjects:
Animals
Biological Sciences
Development Biology
DNA - metabolism
DNA Methylation
Embryos
Female
Gene Expression Regulation, Developmental - physiology
Genomes
Hybridity
Intracytoplasmic sperm injections
Life Sciences
Male
Methylation
Mice
Microscopy, Confocal
Oocytes
Oocytes - metabolism
Pronucleus
Reproductive Biology
Sheep
Sheep - genetics
Sperm Injections, Intracytoplasmic
Spermatozoa
Spermatozoa - metabolism
Ungulates
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Summary:In contrast to mice, in sheep no genome-wide demethylation of the paternal genome occurs within the first postfertilization cell cycle. This difference could be due either to an absence of a sheep demethylase activity that is present in mouse ooplasm or to an increased protection of methylated cytosine residues in sheep sperm. Here, we use interspecies intracytoplasmic sperm injection to demonstrate that sheep sperm DNA can be demethylated in mouse oocytes. Surprisingly, mouse sperm can also be demethylated to a limited extent in sheep oocytes. Our results suggest that the murine demethylation process is facilitated either by a sperm-derived factor or by male pronuclear chromatin composition.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0400730101