MS14 down-regulates lipocalin2 expression in spinal cord tissue in an animal model of multiple sclerosis in female C57BL/6

• Published in: Iranian biomedical journal 2014, Vol.18 (4), p.196-202
• Main Authors: Ebrahimi-Kalan, Abbas, Soleimani Rad, Jafar, Kafami, Laya, Mohammadnejad, Daryoush, Habibi Roudkenar, Mehryar, Khaki, Amir Afshin, Aliyari Serej, Zeynab, Mohammadi Roushandeh, Amaneh
• Format: Article
• Language: English
• Subjects: Acute-Phase Proteins - genetics; Acute-Phase Proteins - metabolism; Animals; Anti-Inflammatory Agents - pharmacology; Disease Models, Animal; Down-Regulation - drug effects; Encephalomyelitis, Autoimmune, Experimental - drug therapy; Encephalomyelitis, Autoimmune, Experimental - genetics; Encephalomyelitis, Autoimmune, Experimental - metabolism; Female; Immunologic Factors - pharmacology; Lipocalin-2; Lipocalins - genetics; Lipocalins - metabolism; Mice; Mice, Inbred C57BL; Multiple Sclerosis - drug therapy; Multiple Sclerosis - genetics; Multiple Sclerosis - metabolism; Oncogene Proteins - genetics; Oncogene Proteins - metabolism; Original; Phytotherapy; Plant Preparations - pharmacology; Spinal Cord - drug effects; Spinal Cord - metabolism; Spinal Cord - pathology
• ISSN: 1028-852X; 2008-823X
• DOI: 10.6091/ibj.1375.2014

Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis, which is a demyelinating and an inflammatory disease of central nervous system. Recent studies have established that some molecules such as Lipocaline2 (LCN2), which expresses during inflammatory conditions, play an important role in EAE pathogenesis and might involve in its treatment process. Recently, it has been proved that MS14, an herbal-marine drug, has anti-inflammatory properties through reduction of TNF-α and IL-1β. Thus, the present study investigated the effects of MS14 on the course of EAE and its relation to LCN2 expression in both protein and gene levels. EAE was induced in female C57BL/6 mice using Hooke kits. Animals were scored for clinical signs of the disease according to a 10-point EAE scoring system. On 21st and 35th days after immunization, mice (n = 4/group) were deeply anesthetized, and the spinal cords were removed. Inflammatory cell infiltration and LCN2 expression in spinal cord were assessed by hematoxylin and eosin staining, immuno-histochemistry, and real-time PCR methods. MS14 significantly ameliorated EAE symptoms and decreased lymphocyte infiltration into the spinal cord (P