Temperature profiles of different cooling methods in porcine pancreas procurement

Porcine islet xenotransplantation is a promising alternative to human islet allotransplantation. Porcine pancreas cooling needs to be optimized to reduce the warm ischemia time (WIT) following donation after cardiac death, which is associated with poorer islet isolation outcomes. This study examines...

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Bibliographic Details
Published in:Xenotransplantation (Københaven) 2014-11, Vol.21 (6), p.574-581
Main Authors: Weegman, Bradley P., Suszynski, Thomas M., Scott III, William E., Ferrer Fábrega, Joana, Avgoustiniatos, Efstathios S., Anazawa, Takayuki, O'Brien, Timothy D., Rizzari, Michael D., Karatzas, Theodore, Jie, Tun, Sutherland, David E. R., Hering, Bernhard J., Papas, Klearchos K.
Format: Article
Language:English
Subjects:
Animals
Cell Separation - methods
cold preservation
Cold Temperature
Humans
islet xenotransplantation
Islets of Langerhans - cytology
Islets of Langerhans Transplantation - methods
organ cooling
Pancreas - cytology
pancreas procurement
Swine
Transplantation, Heterologous - methods
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Summary:Porcine islet xenotransplantation is a promising alternative to human islet allotransplantation. Porcine pancreas cooling needs to be optimized to reduce the warm ischemia time (WIT) following donation after cardiac death, which is associated with poorer islet isolation outcomes. This study examines the effect of four different cooling Methods on core porcine pancreas temperature (n = 24) and histopathology (n = 16). All Methods involved surface cooling with crushed ice and chilled irrigation. Method A, which is the standard for porcine pancreas procurement, used only surface cooling. Method B involved an intravascular flush with cold solution through the pancreas arterial system. Method C involved an intraductal infusion with cold solution through the major pancreatic duct, and Method D combined all three cooling Methods. Surface cooling alone (Method A) gradually decreased core pancreas temperature to
ISSN:0908-665X
1399-3089
1399-3089
DOI:10.1111/xen.12114